Redescription of Onchocerca lupi (Spirurida: Onchocercidae) with histopathological observations

Particle and Fibre Toxicology

Domenico Otranto⁵  Helder Cortes³  Luís Cardoso²  Elias Papadopoulos⁶  Francesca Abramo⁴  Alessio Giannelli⁵  Filipe Dantas-Torres⁵  Yasen Mutafchiev¹ 

[1] Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria;Parasite Disease Group, Instituto de Biologia Molecular e Celular, Universidade do Porto, Oporto, Portugal;Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Évora, Portugal;Dipartimento di Patologia Animale, Università di Pisa, Pisa, Italy;Dipartimento di Medicina Veterinaria, Università degli Studi di Bari, Valenzano, Bari, Italy;Department of Infectious and Parasitic Diseases and Pathology, Faculty of School of Veterinary Medicine, Aristotle, University of Thessaloniki, Thessaloniki, Greece

关键词: Zoonosis;    Histology;    Light microscopy;    Scanning electron microscopy;    Vector-borne disease;    Onchocerca lupi;   

Others  :  824108
DOI  :  10.1186/1756-3305-6-309

 received in 2013-09-23, accepted in 2013-10-21,  发布年份 2013

【 摘 要 】

Background

Onchocerca lupi is a dog parasite of increasing zoonotic concern, with new human cases diagnosed in Turkey, Tunisia, Iran, and the United States. Information about the morphology of this nematode is scant and a detailed re-description of this species is overdue. In addition, histopathological data of potential usefulness for the identification of O. lupi infections are provided.

Methods

Male and female nematodes, collected from the connective tissue of a dog, were examined using light microscopy and scanning electron microscopy (SEM), and an histological evaluation was performed on biopsy samples from periocular tissues.

Results

The morphological identification was confirmed by molecular amplification and partial sequencing of cytochrome oxidase subunit 1 gene. This study provides the first comprehensive morphological and morphometric description of O. lupi from a dog based on light microscopy, SEM, molecular characterization, and histological observations.

Conclusions

Data herein presented contribute to a better understanding of this little known parasitic zoonosis, whose impact on human and animal health is still underestimated. The presence of granulomatous reactions only around the female adult suggests that the release of microfilariae from the uterus might be the cause of the inflammatory reaction observed.

【 授权许可】

   
2013 Mutafchiev et al.; licensee BioMed Central Ltd.

【 图 表 】

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