Adhesion molecules in subjects with COPD and healthy non-smokers: a cross sectional parallel group study

Respiratory Research

Barbro Dahlén²  Kjell Larsson³  Ann-Sofie Lantz²  Elisabeth Henriksson²  Bo Billing²  Anna James¹  Lena Palmberg³  Kristin Blidberg³ 

[1] Experimental Asthma and Allergy research, Division of Physiology, The National Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden;Division of Respiratory Medicine and Allergy, Dept of Medicine, Karolinska University Hospital Huddinge, Stockholm, Sweden;Lung and Allergy Research, National Institute of Environmental Medicine, Karolinska Institutet, Box 287, Stockholm, SE-171 77, Sweden

关键词: Bronchoialveolar lavage fluid;    Sputum;    Neutrophils;    Chronic Obstructive Pulmonary Disease;    Adhesion molecules;   

Others  :  794722
DOI  :  10.1186/1465-9921-14-47

 received in 2012-11-07, accepted in 2013-04-24,  发布年份 2013

【 摘 要 】

Background

The aim of the study was to investigate how the expression of adhesion molecules changes as neutrophils migrate from the circulation to the lung and if these changes differ between non-smoking subjects and smokers with and without COPD.

Methods

Non-smoking healthy subjects (n=22), smokers without (n=21) and with COPD (n=18) were included. Neutrophils from peripheral blood, sputum and bronchial biopsies were analysed for cell surface expression of adhesion molecules (CD11b, CD62L, CD162). Serum, sputum supernatant and BAL-fluid were analysed for soluble adhesion molecules (ICAM-1, -3, E-selectin, P-selectin, VCAM-1, PECAM-1).

Results

Expression of CD11b was increased on circulating neutrophils from smokers with COPD. It was also increased on sputum neutrophils in both smokers groups, but not in non-smokers, as compared to circulating neutrophils.

Serum ICAM-1 was higher in the COPD group compared to the other two groups (p<0.05) and PECAM-1 was lower in smokers without COPD than in non-smoking controls and the COPD group (p<0.05). In BAL-fluid ICAM-1 was lower in the COPD group than in the other groups (p<0.05).

Conclusions

Thus, our data strongly support the involvement of a systemic component in COPD and demonstrate that in smokers neutrophils are activated to a greater extent at the point of transition from the circulation into the lungs than in non-smokers.

【 授权许可】

   
2013 Blidberg et al.; licensee BioMed Central Ltd.

【 图 表 】

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