Characteristics of epstein barr virus variants associated with gastric carcinoma in Southern Tunisia

Virology Journal

Raja Mokdad-Gargouri¹  Ali Gargouri¹  Tahia Sellami-Boudawara²  Abdelmajid Khabir²  Imen Miladi-Abdennadher¹  Wajdi Ayadi¹  Dorra BenAyed-Guerfali¹ 

[1] Department of Molecular Genetics of Eucaryotes, Center of Biotechnology of Sfax, University of Sfax, Sidi Mansour Street, Sfax 301, Tunisia;Department of Anatomo-pathology, Habib Bourguiba Hospital, El Ain Street, Sfax 3000, Tunisia

关键词: polymorphisms;    EBER;    EBV;    Gastric carcinoma;   

Others  :  1155707
DOI  :  10.1186/1743-422X-8-500

 received in 2011-06-15, accepted in 2011-11-03,  发布年份 2011

【 摘 要 】

Backgroud

EBV-associated Gastric Carcinoma (EBVaGC) has a distinct clinical features and its prevalence is variable worldwide.

Results

To determine the prevalence of EBVaGC in Tunisia, EBV-encoded small RNA (EBER) expression was assessed in 81 gastric carcinoma (GC) specimens. The nuclear EBER expression was detected in 12 out of 81 GC cases (14.81%) and concordance between the score range of EBER staining and the number of EBV DNA copies as estimate by QPCR is observed. On the other hand, we found that EBVaGC strongly correlated with age at diagnosis, and weakly with tumor differentiation and venous invasion.

Furthermore, the EBVaGC specimens were subjected to determine the EBV DNA polymorphisms. Our results show a unique genetic profile of the EBV strains regarding the A and D types, the F prototype, the retention of XhoI restriction site and the 30 bp del-LMP1 variant. According to our previous studies on nasopharyngeal carcinoma (NPC), we suggested that EBV strains associated to GC and NPC shared some similarities in Tunisian patients.

Conclusion

The prevalence of EBVaGC is of 14.81% in the southern Tunisia and that common EBV strain are associated with both NPC and GC which are likely to differ from Asian strains. Our findings support therefore a certain geographical distribution of EBV strains which is not restricted to EBV-associated malignancies.

【 授权许可】

   
2011 BenAyed-Guerfali et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

【 参考文献 】

• [1]Correa P, Piazuelo MB, Camargo MC: The future of gastric cancer prevention. Gastric Cancer 2004, 7:9-16.
• [2]Rocco A, Staibano S, Ottini L, Mezza E, Somma P, Mariani-Costantini R: Is there a link between environmental factors and genetic predisposition to cancer? A lesson from a familial cluster of gastric cancer. Eur J Cancer 2003, 39:1619-1624.
• [3]Yustein AS, Harper JC, Petroni GR, Cummings OW, Moskaluk CA, Powell SM: Allelotype of gastric adenocarcinoma. Cancer Resv 1999, 59:1437-1441.
• [4]Hsairi M, Fakfakh R, Ben Abdallah M, Jelidi R, Sellami A, Nacef T: Assessment of cancer incidence in Tunisia. La Tunisie Médicale 2002, 80:57-64.
• [5]Fukayama M, shiku T: Epstein Barr virus associated gastric carcinoma. Pathol Res and Pract 2011, 207:529-537.
• [6]Lee JH, Kim SH, Han SH, An JS, Lee ES, Kim YS: Clinicopathological and molecular characteristics of Epstein-Barr virus-associated gastric carcinoma: a meta-analysis. J Gastroenterol Hepatol 2009, 24:354-365.
• [7]Osato T, Imai S: Epstein-Barr virus and gastric carcinoma. Semin Cancer Biol 1996, 7:175-182.
• [8]Tokunaga M, Land CE, Uemura Y, Tokudome T, Tanaka S, Sato E: Epstein-Barr virus in gastric carcinoma. Am J Pathol 1993, 143:1250-1254.
• [9]Uozaki H, Fukayama M: Epstein-Barr virus associated gastric carcinoma-viral carcinogenesis through epigenetic mechanisms. Int J Clin Exp Pathol 2008, 1:198-216.
• [10]Takada K, Nanbo A: The role of EBERs in oncogenesis. Semin Cancer Biol 2001, 11:461-467.
• [11]Imai S, Koizumi S, Sugiura M, Tokunaga M, Uemura Y, Yamamoto N, Tanaka S, Sato E, Osato T: Gastric carcinoma: monoclonal epithelial malignant cells expressing Epstein-Barr virus latent infection protein. Proc Natl Acad Sci USA 1994, 91:9131-9135.
• [12]Shinkura R, Yamamoto N, Koriyama C, Shinmura Y, Eizuru Y, Tokunaga M: Epstein-Barr virus-specific antibodies in Epstein-Barr virus-positive and -negative gastric carcinoma cases in Japan. J Med Virol 2000, 60:411-416.
• [13]Abdirad A, Ghaderi-Sohi S, Shuyama K, Koriyama C, Nadimi-Barforoosh H, Emami S, Mosavi-Jarrahi A, Nahvijou A, Akiba S: Epstein-Barr virus associated gastric carcinoma: a report from Iran in the last four decades. Diagn Pathol 2007, 15:22-25.
• [14]Alipov G, Nakayama T, Nakashima M, Wen CY, Niino D, Kondo H, Pruglo Y, Sekine I: Epstein-Barr virus-associated gastric carcinoma in Kazakhstan. World J Gastroenterol 2005, 11:27-30.
• [15]Burgess DE, Woodman CB, Flavell KJ, Rowlands DC, Crocker J, Scott K, Biddulph JP, Young LS, Murray PG: Low prevalence of Epstein-Barr virus in incident gastric adenocarcinomas from the United Kingdom. Br J Cancer 2002, 86:702-704.
• [16]Chang MS, Lee HS, Kim CW, Kim YI, Kim WH: Clinicopathologic characteristics of Epstein-Barr virus-incorporated gastric cancers in Korea. Pathol Res Pract 2001, 197:395-400.
• [17]Corvalan A, Koriyama C, Akiba S, Backhouse C, Palma M, Argandoña J, Tokunaga M: Epstein-Barr virus in gastric carcinoma is associated with location in the cardia and with a diffuse histology: a study in one area of Chile. Int J Cancer 2001, 94:527-530.
• [18]Hao Z, Koriyama C, Akiba S, Li J, Luo X, Itoh T, Eizuru Y, Zou J: The Epstein-Barr virus- associated gastric carcinoma in Southern and Northern China. Oncol Rep 2002, 9:1293-1298.
• [19]Herrera-Goepfert R, Akiba S, Koriyama C, Ding S, Reyes E, Itoh T, Minakami Y, Eizuru Y: Epstein-Barr virus-associated gastric carcinoma: evidence of age-dependant among a Mexican population. W J Gastroenterol 2005, 11:6096-6103.
• [20]Karim N, Pallesen G: Epstein-Barr virus (EBV) and gastric carcinoma in Malaysian patients. J Pathol 2003, 25:45-47.
• [21]Murphy G, Pfeiffer R, Camargo MC, Rabkin CS: Meta-analysis shows that prevalence of Epstein-Barr virus-positive gastric cancer differs based on sex and anatomic location. Gastroenterology 2009, 137:824-833.
• [22]Fukayama M, Hino R, Uozaki H: Epstein-Barr virus and gastric carcinoma: virus-host interactions leading to carcinoma. Cancer Sci 2008, 99:1726-1733.
• [23]Zur Hausen A, Brink AA, Craanen ME, Middeldorp JM, Meijer CJ, Van Den Brule AJ: Unique transcription pattern of Epstein-Barr virus (EBV) in EBV-carrying gastric adenocarcinomas: expression of the transforming BARF1 gene. Cancer Res 2000, 60:2745-2748.
• [24]Lauren P: The two histological main types of gastric carcinoma: diffuse and so-called intestinal-type carcinoma. An attempt at a histo-clinical classification. Acta Pathol Microbiol Scand 1965, 64:31-49.
• [25]Sobin LH, Fleming ID: TNM classification of malignant tumors, fifth edition Union. Internationale Contre le Cancer and the American Joint Committee on Cancer. Cancer 1997, 80:1803-1804.
• [26]Sambrook J, Russell DW: Molecular cloning. A laboratory manual, Cold Spring Harbor, NY; Cold Spring Harbor Laboratory Press; 2001.
• [27]Hu LF, Zabarovsky ER, Chen F, Cao SL, Ernberg I, Klein G, Winberg G: Isolation and sequencing of the Epstein-Barr virus BNLF-1 gene (LMP1) from Chinese nasopharyngeal carcinoma. J Gen Virol 1991, 72:2399-2409.
• [28]Cheung ST, Leung SF, Lo KW, Chiu KW, Tam SJ, Fok TF, Johnson PJ, Lee JC, Huang DP: Specific latent membrane protein 1 gene sequences in type1 and type 2 Epstein Barr virus from nasopharyngeal carcinoma in Hong Kong. Int J Cancer 1998, 76:399-406.
• [29]Hadhri-Guiga B, Khabir AM, Mokdad-Gargouri R, Ghorbel AM, Drira M, Daoud J, Frikha M, Jlidi R, Gargouri A: Various 30 and 69 bp deletion variants of the Epstein-Barr virus LMP1 may arise by homologous recombination in nasopharyngeal carcinoma of Tunisian patients. Virus Res 2006, 115:24-30.
• [30]Lo YM, Chan LY, Chan AT, Leung SF, Lo KW, Zhang J, Lee JC, Hjelm NM, Johnson PJ, Huang DP: Quantitative and temporal correlation between circulating cell-free Epstein-Barr virus DNA and tumor recurrence in nasopharyngeal carcinoma. Cancer Res 1991, 59:5452-5455.
• [31]Yoshiwara E, Koriyama C, Akiba S, Itoh T, Minakami Y, Chirinos JL, Watanabe J, Takano J, Miyagui J, Hidalgo H, Chacon P, Linares V, Eizuru Y: Epstein-Barr virus-associated gastric carcinoma in Lima, Peru. Jpn J Cancer Res 2005, 24:49-54.
• [32]Truong CD, Feng W, Li W, Khoury T, Li Q, Alrawi S, Yu Y, Xie K, Yao J, Tan D: Characteristics of Epstein-Barr virus-associated gastric cancer: a study of 235 cases at a comprehensive cancer center in USA. J Exp Clin Cancer Res 2009, 3(28):14.
• [33]Ryan JL, Morgan DR, Dominguez RL, Thorne LB, Elmore SH, Lauwers GY, Booker JK, Gulley ML: High Levels of Epstein-Barr Virus DNA in Latently Infected Gastric Adenocarcinoma. Lab Invest 2009, 89:80-90.
• [34]Trimeche M, Ksiaa F, Ziadi S, Mestiri S, Hachana M, Ben Gacem R, Sriha B, Korbi S: Prevalence and characteristics of Epstein-Barr virus-associated gastric carcinomas in Tunisia. Eur J Gastro Hepato 2009, 21:1001-1007.
• [35]Chen JN, Ding YG, Feng ZY, Li HG, He D, Du H, Wu B, Shao CK: Asociation of distinctive Epstein-Barr virus variants with gastric carcinoma in Guangzhou, southern China. J Med Virol 2010, 82:658-667.
• [36]Galetsky SA, Tsvetnov VV, Land CE, Afanasieva TA, Petrovichev NN, Gurtsevitch VE, Tokunaga M: Epstein-Barr-virus-associated gastric cancer in Russia. Int J Cancer 1997, 73:786-789.
• [37]Murphy G, Pfeiffer R, Camargo MC, Rabkin CS: Meta-analysis shows that prevalence of Epstein-Barr virus-positive gastric cancer differs based on sex and anatomic location. Gastroenterology 2009, 137:824-833.
• [38]Camargo MC, Murphy G, Koriyama C, Pfeiffer RM, Kim WH, Herrera-Goepfert R, Corvalan AH, Carrascal E, Abdirad A, Anwar M, Hao Z, Kattoor J, Yoshiwara-Wakabayashi E, Eizuru Y, Rabkin CS, Akiba S: Determinants of Epstein-Barr virus-positive gastric cancer: an international pooled analysis. Br J Cancer 2011, 105:38-43.
• [39]Fukayama M, Ushiku T: Epstein-Barr virus-associated gastric carcinoma. Pathol Res and Practice 2011, 207:529-537.
• [40]Sidagis J, Ueno K, Tokunaga M, Ohyama M, Eizuru Y: Molecular epidemiology of Epstein-Barr virus (EBV) in EBV-related malignancies. Int J Cancer 1997, 72:72-76.
• [41]Corvalan A, Ding S, Koriyama C, Carrascal E, Carrasquilla G, Backhouse C, Urzua L, Argandona J, Palma M, Eizuru Y, Akiba S: Association of a distinctive strain of Epstein-Barr virus with gastric carcinoma. Int J Cancer 2006, 118:1736-1742.
• [42]Lung ML, Lam WP, Sham J, Choy D, Yong-Sheng Z, Guo HY, Ng MH: Detection and prevalence of the ''f'' variant of Epstein-Barr virus in southern China. Virology 1991, 185:67-71.
• [43]Ayadi W, Feki L, Khabir A, Boudawara T, Ghorbel A, Charfeddine I, Daoud J, Frikha M, Hammami A, Karray-Hakim H: Polymorphism analysis of Epstein-Barr virus isolates of nasopharyngeal carcinoma biopsies from Tunisian patients. Virus Genes 2007, 34:137-145.
• [44]Edwards RH, Sitki-Green D, Moore D T, Raab-Traub N: Potential Selection of LMP1 Variants in Nasopharyngeal Carcinoma. J Virol 2004, 78:868-881.