Epstein-Barr Virus EBER Transcripts Affect miRNA-Mediated Regulation of Specific Targets and Are Processed to Small RNA Species

Non-Coding RNA

Julia Alles³  Daniele Hasler¹  Syed Mohammad Ali Kazmi⁴  Mathias Tesson⁴  Andrew Hamilton⁴  Linda Schlegel³  Stefanie Marx³  Norbert Eichner¹  Richard Reinhardt²  Gunter Meister¹  Joanna B. Wilson⁴  Friedrich A. Grässer³ 

[1] Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, Universitätsstrasse 31, Regensburg D-93053, Germany; E-Mails:;Max Planck Genome Centre Cologne, Carl-von-Linné-Weg 10, Cologne D-50829, Germany; E-Mail:;Institute of Virology, Saarland University Medical School, Kirrbergerstrasse, Haus 47, Homburg/Saar D-66421, Germany; E-Mails:;College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK; E-Mails:

关键词: Epstein-Barr virus;    EBV;    EBER;    microRNA;    DICER;    Lupus antigen;    La;    ebv-miR-BART16;    hsa-miR-142;    IL1A;    RAC1;    TOMM22;    ADCY9;   

DOI  :  10.3390/ncrna1030170

来源: mdpi

【 摘 要 】

The oncogenic Epstein-Barr virus (EBV) expresses 44 mature microRNAs and two non-coding EBER RNAs of 167 (EBER1) and 172 (EBER2) nt length. MiRNA profiling of NK/T cell lines and primary cells and Northern blotting of EBV-infected cell lines and primary tumors revealed processing of EBER1 to short 5′-derived RNAs of approximately 23, 52 and 70 nt (EBER1₂₃, EBER1₅₂, and EBER1₇₀) and of EBER2 to 3′ fragments. The biogenesis of these species is independent of Dicer, and EBER1₂₃ does not act like a miRNA to target its complementary sequence. EBER1, EBER2 and EBER1₂₃ were bound by the lupus antigen (La), a nuclear and cytoplasmic protein that facilitates RNAi. Consistent with this, the EBERs affect regulation of interleukin 1alpha (IL1α) and RAC1 reporters harboring miR target sequences, targets of miR-142-3p. However, the EBERs have no effect upon another target of miR-142-3p, ADCY9, nor on TOMM22, a target of ebv-miR-BART16, indicative of selective modulation of gene expression by the EBERs.

【 授权许可】

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