Genome-wide comparative analysis revealed significant transcriptome changes in mice after Toxoplasma gondii infection

Particle and Fibre Toxicology

Qijun Chen³  Ning Jiang¹  Jigang Yin¹  Quan Liu²  Huijun Lu¹  Boyin Jia¹ 

[1] Key Laboratory of Zoonosis, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, China;Institute of Military Veterinary, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, 666 Liuying Xilu, Changchun 130122, Jilin Province, China;MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, Dongdan Santiao 9, Beijing 100730, China

关键词: Peripheral lymphocytes;    Brain tissues;    Gene ontology and pathway analysis;    Microarray;    ME49 strain;    RH strain;    Toxoplasma gondii;   

Others  :  1227020
DOI  :  10.1186/1756-3305-6-161

 received in 2013-02-24, accepted in 2013-05-19,  发布年份 2013

【 摘 要 】

Background

Toxoplasma gondii is an intracellular parasite that can modulate host responses and presumably host behavior. Host responses as well as pathogenesis vary depending on the parasite strains that are responsible for infection. In immune competent individuals, T. gondii preferentially infects tissues of the central nervous systems (CNS), which might be an additional factor in certain psychiatric disorders. While in immune-compromised individuals and pregnant women, the parasite can cause life-threatening infections. With the availability of the genome-wide investigation platform, the global responses in gene expression of the host after T. gondii infection can be systematically investigated.

Methods

Total RNA of brain tissues and peripheral lymphocytes of BALB/C mice infected with RH and ME 49 strain T. gondii as well as that of healthy mice were purified and converted to cRNA with incorporated Cy5-CTP (experimental samples), or Cy3-CTP (control samples). The labeled cRNA probes were hybridized to the Whole Mouse Genome Microarray. The impact of parasite infection on gene expression in both brain tissues and peripheral lymphocytes were analyzed. Differentially expressed genes were revalidated with real-time quantitative reverse transcriptase-polymerase chain reaction (Q-PCR).

Results

Data indicated that the genes associated with immunity were up-regulated after infection by the two parasite strains, but significant up-regulation was observed in both brain tissues and peripheral lymphocytes of mice infected with ME49 strain compared to that infected by RH strain. The pathways related to pathogenesis of the nervous system were more significantly up-regulated in mice infected with RH strain.

Conclusions

Genetically distinct T. gondii strains showed clear differences in modulation of host pathophysiological and immunological responses in both brain tissue and peripheral lymphocytes. It was likely that some of the host responses to T. gondii infection were universal, but the immune response and CNS reaction were in a strain-specific manner.

【 授权许可】

   
2013 Jia et al.; licensee BioMed Central Ltd.

【 图 表 】

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