Arsenic affects inflammatory cytokine expression in Gallus gallus brain tissues

BMC Veterinary Research

Research Article

Siwen Li¹  Mingwei Xing¹  Ying He¹  Hongjing Zhao¹  Ying Guo¹  Yu Wang¹  Xiao Sun¹  Jingyu Zhang¹ 

[1] College of Wildlife Resources, Northeast Forestry University, 150040, Harbin, Heilongjiang Province, China;

关键词: Arsenic;    NF-κB;    Inflammatory cytokines;    Brain tissues;    Chickens;   

DOI  :  10.1186/s12917-017-1066-8

 received in 2016-09-19, accepted in 2017-05-18,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe heavy metal arsenic is widely distributed in nature and posses a serious threat to organism’s health. However, little is known about the arsenic-induced inflammatory response in the brain tissues of birds and the relationship and mechanism of the inflammatory response. The purpose of this study was to explore the effects of dietary arsenic on the expression of inflammatory cytokines in the brains of Gallus gallus.ResultsSeventy-two 1-day-old male Hy-line chickens were divided into a control group, a low arsenic trioxide (As2O3)-treated (7.5 mg/kg) group, a middle As2O3-treated (15 mg/kg) group, and a high As2O3-treated (30 mg/kg) group. Arsenic exposure caused obvious ultrastructural changes. The mRNA levels of the transcription factor nuclear factor-κB (NF-κB) and of pro-inflammatory cytokines, including inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and prostaglandin E synthase (PTGEs), in chicken brain tissues (cerebrum, cerebellum, thalamus, brainstem and myelencephalon) on days 30, 60 and 90, respectively, were measured by real-time PCR. The protein expression of iNOS was detected by western blot. The results showed that after being treated with As2O3, the levels of inflammatory-related factor NF-κB and pro-inflammatory cytokines in chicken brain tissues increased (P < 0.05).ConclusionsArsenic exposure in the chickens triggered host defence and induced an inflammatory response by regulating the expression of inflammatory-related genes in the cerebrum, cerebellum, thalamus, brainstem and myelencephalon. These data form a foundation for further research on arsenic-induced neurotoxicity in Gallus gallus.

【 授权许可】

CC BY   
© The Author(s). 2017