Comparative secretome analysis of four isogenic Bacillus clausii probiotic strains

Proteome Science

Sergio Papa¹  Anna Maria Sardanelli¹  Antonio Gnoni¹  Anna Abbrescia¹  Maria Fiorella Mazzeo³  Rosa Anna Siciliano³  Rosa Lippolis² 

[1] Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari, Policlinico, Piazza G. Cesare, 70124, Bari, Italy;Institute of Biomembranes and Bioenergetics, Italian National Research Council (CNR), Via Amendola 165/A, Bari, Italy;Institute of Food Sciences, Italian National Research Council (CNR), Via Roma, 64, 83100, Avellino, Italy

关键词: Mass Spectrometry;    Two-dimensional Gel Electrophoresis;    Proteomics;    Secretome;    Probiotics;    Bacillus clausii;   

Others  :  816866
DOI  :  10.1186/1477-5956-11-28

 received in 2012-11-14, accepted in 2013-06-07,  发布年份 2013

【 摘 要 】

Background

The spore-bearing alkaliphilic Bacillus species constitute a large, heterogeneous group of microorganisms, important for their ability to produce enzymes, antibodies and metabolites of potential medical use. Some Bacillus species are currently being used for manufacturing probiotic products consisting of bacterial spores, exhibiting specific features (colonization, immune-stimulation and antimicrobial activity) that can account for their claimed probiotic properties. In the present work a comparative proteomic study was performed aimed at characterizing the secretome of four closely related isogenic O/C, SIN, N/R and T B. clausii strains, already marketed in a pharmaceutical mixture as probiotics.

Results

Proteomic analyses revealed a high degree of concordance among the four secretomes, although some proteins exhibited considerable variations in their expression level in the four strains. Among these, some proteins with documented activity in the interaction with host cells were identified, such as the glycolytic enzyme enolase, with a putative plasminogen-binding activity, GroEL, a molecular chaperone shown to be able to bind to mucin, and flagellin protein, a structural flagella protein and a putative immunomodulation agent.

Conclusion

This study shows, for the first time, differences in the secretome of the OC, SIN, NR and T B. clausii strains. These differences indicate that specific secretome features characterize each of the four strains despite their genotypic similarity. This could confer to the B. clausii strains specific probiotic functions associated with the differentially expressed proteins and indicate that they can cooperate as probiotics as the secretome components of each strain could contribute to the overall activity of a mixed probiotic preparation.

【 授权许可】

   
2013 Lippolis et al.; licensee BioMed Central Ltd.

【 图 表 】

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