Development and application of an antigen capture ELISA assay for diagnosis of Japanese encephalitis virus in swine, human and mosquito

Virology Journal

Shengbo Cao¹  Huanchun Chen¹  Xiaohong Yang¹  Yaoming Li¹  Shaomei Huang¹  Lin Shao¹  Guangping Gao³  Jing Ye¹  Peng Wu²  Li Mei¹ 

[1] Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China;Department of Clinical Laboratory, Taihe Hospital, Hubei University of Medicine, 16 Shiyan, Shiyan 442000, China;Department of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066600, China

关键词: Antigen capture ELISA;    Polyclonal antibody;    Monoclonal antibody;    Japanese encephalitis virus;    Japanese encephalitis;   

Others  :  1155317
DOI  :  10.1186/1743-422X-9-4

 received in 2011-10-03, accepted in 2012-01-06,  发布年份 2012

【 摘 要 】

Background

Japanese encephalitis (JE) is a serious zoonosis caused by the Japanese encephalitis virus (JEV) which is a mosquito-borne pathogen of the family Flavivirus. However, the application of several developed laboratory methods for the detection of JEV antigens or antibodies are limited by their requirements of laboratory operations, skilled technicians and special facilities.

Results

To develop a method for detecting JEV antigen in swine, human, mosquito and other clinical specimens specifically, conveniently and effectively, an antigen capture enzyme-linked immunosorbent assay (ELISA) was established in this study. Sensitivity, specificity, repeatability and stability of the developed method were evaluated, and 60 clinical samples were tested in this study. The results demonstrated that the antigen capture ELISA was capable in detecting JEV antigen with high sensitivity and specificity compared with conventional methods. 14 samples showed the positive result with coincidence rate of 70%, and 46 displayed negative result with coincidence rate of 100% as compared to that of reverse transcription-polymerase chain reaction (RT-PCR).

Conclusions

The developed ELISA assay provides a convenient and specific method for the large-scale determination of JEV antigen in infected swine, human and mosquito samples with high sensitivity and specificity.

【 授权许可】

   
2011 Mei et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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