期刊论文详细信息
Journal of Biological Engineering
“NiCo Buster”: engineering E. coli for fast and efficient capture of cobalt and nickel
Corinne Dorel3  Agnès Rodrigue2  Valérie Desjardin3  Fanny Springer3  Philippe Lejeune2  Yoann Louis3  Clémence Gonthier1  Franck Frémion1  Viviane Chansavang1  Alexandre Duprey2 
[1] iGEM team INSA Lyon, Plateforme de Biologie de Synthèse, Département Biosciences, INSA Lyon, 69621 Villeurbanne Cedex, France;Université de Lyon, INSA-Lyon, Université Claude Bernard Lyon I, CNRS, MAP, UMR5240, Villeurbanne F-69621, France;Université de Lyon, INSA-Lyon, LGCIE, Villeurbanne F-69621, France
关键词: Synthetic biology;    NiCoT;    Biofilter;    Biofilm;    Nickel;    Cobalt;    Bioremediation;   
Others  :  1135900
DOI  :  10.1186/1754-1611-8-19
 received in 2014-02-18, accepted in 2014-07-13,  发布年份 2014
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【 摘 要 】

Background

Metal contamination is widespread and results from natural geogenic and constantly increasing anthropogenic sources (mainly mining and extraction activities, electroplating, battery and steel manufacturing or metal finishing). Consequently, there is a growing need for methods to detoxify polluted ecosystems. Industrial wastewater, surface water and ground water need to be decontaminated to alleviate the contamination of soils and sediments and, ultimately, the human food chain. In nuclear power plants, radioactive metals are produced; these metals need to be removed from effluents before they are released into the environment, not only for pollution prevention but also for waste minimization. Many physicochemical methods have been developed for metal removal from aqueous solutions, including chemical coagulation, adsorption, extraction, ion exchange and membrane separation; however, these methods are generally not metal selective. Bacteria, because they contain metal transporters, provide a potentially competitive alternative to the current use of expensive and high-volume ion-exchange resins.

Results

The feasibility of using bacterial biofilters as efficient tools for nickel and cobalt ions specific remediation was investigated. Among the factors susceptible to genetic modification in Escherichia coli, specific efflux and sequestration systems were engineered to improve its metal sequestration abilities. Genomic suppression of the RcnA nickel (Ni) and cobalt (Co) efflux system was combined with the plasmid-controlled expression of a genetically improved version of a specific metallic transporter, NiCoT, which originates from Novosphingobium aromaticivorans. The resulting strain exhibited enhanced nickel (II) and cobalt (II) uptake, with a maximum metal ion accumulation of 6 mg/g bacterial dry weight during 10 min of treatment. A synthetic adherence operon was successfully introduced into the plasmid carrying the improved NiCoT transporter, conferring the ability to form thick biofilm structures, especially when exposed to nickel and cobalt metallic compounds.

Conclusions

This study demonstrates the efficient use of genetic engineering to increase metal sequestration and biofilm formation by E. coli. This method allows Co and Ni contaminants to be sequestered while spatially confining the bacteria to an abiotic support. Biofiltration of nickel (II) and cobalt (II) by immobilized cells is therefore a promising option for treating these contaminants at an industrial scale.

【 授权许可】

   
2014 Duprey et al.; licensee BioMed Central Ltd.

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【 参考文献 】
  • [1]Besser JM, Leib KJ: Toxicity of metals in water and sediment to aquatic biota. In Integr Investig Environ Eff Hist Min Animas River Watershed S Juan Cty Colo Edited by Church SE, von Guerard P, Finger SE. 2007.
  • [2]Ahluwalia SS, Goyal D: Microbial and plant derived biomass for removal of heavy metals from wastewater. Bioresour Technol 2007, 98:2243-2257.
  • [3]Raghu G, Balaji V, Venkateswaran G, Rodrigue A, Maruthi Mohan P: Bioremediation of trace cobalt from simulated spent decontamination solutions of nuclear power reactors using E. coli expressing NiCoT genes. Appl Microbiol Biotechnol 2008, 81:571-578.
  • [4]Mustafa YA, Zaiter MJ: Treatment of radioactive liquid waste (Co-60) by sorption on Zeolite Na-A prepared from Iraqi kaolin. J Hazard Mater 2011, 196:228-233.
  • [5]Volesky B: Detoxification of metal-bearing effluents: biosorption for the next century. Hydrometallurgy 2001, 59:203-216.
  • [6]Lloyd JR: Bioremediation of metals; the application of micro-organisms that make and break minerals. Interactions 2002, 2:M2.
  • [7]Soares EV, Soares HMVM: Cleanup of industrial effluents containing heavy metals: a new opportunity of valorising the biomass produced by brewing industry. Appl Microbiol Biotechnol 2013, 97:6667-6675.
  • [8]Ruiz ON, Alvarez D, Gonzalez-Ruiz G, Torres C: Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase. BMC Biotechnol 2011, 11:82. BioMed Central Full Text
  • [9]Macaskie LE, Empson RM, Cheetham AK, Grey CP, Skarnulis AJ: Uranium bioaccumulation by a Citrobacter sp. as a result of enzymically mediated growth of polycrystalline HUO2PO4. Science 1992, 257:782-784.
  • [10]Roane TM, Rensing C, Peper IL, Maier RM: Microorganism and metal pollutants. In Environmental Microbiology (2d edition), chapter 21. Edited by Maier RM, Pepper IL, Gerba CP. Academic Press; 2009:421-441. http://www.sciencedirect.com/science/book/9780123705198 webcite
  • [11]Kulkarni S, Ballal A, Apte SK: Bioprecipitation of uranium from alkaline waste solutions using recombinant Deinococcus radiodurans. J Hazard Mater 2013, 262:853-861.
  • [12]Monier M, Ayad DM, Wei Y, Sarhan AA: Adsorption of Cu(II), Co(II), and Ni(II) ions by modified magnetic chitosan chelating resin. J Hazard Mater 2010, 177:962-970.
  • [13]Navarro C, Wu L-F, Mandrand-Berthelot M-A: The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel. Mol Microbiol 1993, 9:1181-1191.
  • [14]Hebbeln P, Eitinger T: Heterologous production and characterization of bacterial nickel/cobalt permeases. FEMS Microbiol Lett 2004, 230:129-135.
  • [15]Rodionov DA, Hebbeln P, Gelfand MS, Eitinger T: Comparative and functional genomic analysis of prokaryotic nickel and cobalt uptake transporters: evidence for a novel group of ATP-binding cassette transporters. J Bacteriol 2006, 188:317-327.
  • [16]Mulrooney SB, Hausinger RP: Nickel uptake and utilization by microorganisms. FEMS Microbiol Rev 2003, 27:239-261.
  • [17]Deng X, He J, He N: Comparative study on Ni2 + −affinity transport of nickel/cobalt permeases (NiCoTs) and the potential of recombinant Escherichia coli for Ni2+ bioaccumulation. Bioresour Technol 2013, 130:69-74.
  • [18]Zhang Y-M, Yin H, Ye J-S, Peng H, Zhang N, Qin H-M, Yang F, He B-Y: Cloning and expression of the nickel/cobalt transferase gene in E. coli BL21 and bioaccumulation of nickel ion by genetically engineered strain. Huan Jing Ke Xue Huanjing Kexue Bian Ji Zhongguo Ke Xue Yuan Huan Jing Ke Xue Wei Yuan Hui Huan Jing Ke Xue Bian Ji Wei Yuan Hui 2007, 28:918-923.
  • [19]Krishnaswamy R, Wilson DB: Construction and characterization of an escherichia coli strain genetically engineered for Ni(II) bioaccumulation. Appl Environ Microbiol 2000, 66:5383-5386.
  • [20]Rodrigue A, Effantin G, Mandrand-Berthelot M-A: Identification of rcnA (yohM), a nickel and cobalt resistance gene in escherichia coli. J Bacteriol 2005, 187:2912-2916.
  • [21]Barnhart MM, Chapman MR: Curli biogenesis and function. Annu Rev Microbiol 2006, 60:131-147.
  • [22]Drogue B, Thomas P, Balvay L, Prigent-Combaret C, Dorel C: Engineering adherent bacteria by creating a single synthetic curli operon. J Vis Exp JoVE 2012, e4176. http://www.jove.com/video/4176/engineering-adherent-bacteria-creating-single-synthetic-curli webcite
  • [23]Blaha D, Arous S, Blériot C, Dorel C, Mandrand-Berthelot M-A, Rodrigue A: The Escherichia coli metallo-regulator RcnR represses rcnA and rcnR transcription through binding on a shared operator site: insights into regulatory specificity towards nickel and cobalt. Biochimie 2011, 93:434-439.
  • [24]Górecka E, Jastrzębska M: Immobilization techniques and biopolymer carriers. Biotechnol Food Sci 2011, 75(nr 1):65-86.
  • [25]Miao H, Ratnasingam S, Pu CS, Desai MM, Sze CC: Dual fluorescence system for flow cytometric analysis of Escherichia coli transcriptional response in multi-species context. J Microbiol Methods 2009, 76:109-119.
  • [26]Perrin C, Briandet R, Jubelin G, Lejeune P, Mandrand-Berthelot M-A, Rodrigue A, Dorel C: Nickel promotes biofilm formation by escherichia coli K-12 strains that produce curli. Appl Environ Microbiol 2009, 75:1723-1733.
  • [27]pSB1C3 is a high copy number plasmid (RFC [10]) carrying chloramphenicol resistance [http://parts.igem.org/Part:pSB1C3?title=Part:pSB1C3 webcite]
  • [28]pSB1T3 is a high copy number plasmid (RFC [10]) carrying tetracycline resistance [http://parts.igem.org/Part:pSB1T3?title=Part:pSB1T3 webcite]
  • [29]Bleriot C, Effantin G, Lagarde F, Mandrand-Berthelot M-A, Rodrigue A: RcnB is a periplasmic protein essential for maintaining intracellular Ni and Co concentrations in escherichia coli. J Bacteriol 2011, 193:3785-3793.
  • [30]Pesavento C, Becker G, Sommerfeldt N, Possling A, Tschowri N, Mehlis A, Hengge R: Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli. Genes Dev 2008, 22:2434-2446.
  • [31]Jubelin G, Vianney A, Beloin C, Ghigo J-M, Lazzaroni J-C, Lejeune P, Dorel C: CpxR/OmpR interplay regulates curli gene expression in response to osmolarity in Escherichia coli. J Bacteriol 2005, 187:2038-2049.
  • [32]Ogasawara H, Yamamoto K, Ishihama A: Role of the biofilm master regulator CsgD in cross-regulation between biofilm formation and flagellar synthesis. J Bacteriol 2011, 193:2587-2597.
  • [33]Wasi S, Tabrez S, Ahmad M: Toxicological effects of major environmental pollutants: an overview. Environ Monit Assess 2013, 185:2585-2593.
  • [34]Nair S, Joshi-Saha A, Singh S, Ramachandran V, Singh S, Thorat V, Kaushik CP, Eapen S, D’Souza SF: Evaluation of transgenic tobacco plants expressing a bacterial Co–Ni transporter for acquisition of cobalt. J Biotechnol 2012, 161:422-428.
  • [35]Brim H, McFarlan SC, Fredrickson JK, Minton KW, Zhai M, Wackett LP, Daly MJ: Engineering Deinococcus radiodurans for metal remediation in radioactive mixed waste environments. Nat Biotechnol 2000, 18:85-90.
  • [36]Rayu S, Karpouzas DG, Singh BK: Emerging technologies in bioremediation: constraints and opportunities. Biodegradation 2012, 23:917-926.
  • [37]Lee J-C, Pandey BD: Bio-processing of solid wastes and secondary resources for metal extraction - a review. Waste Manag 2012, 32:3-18.
  • [38]Miller JH: Experiment in Molecular Genetics. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press; 1972.
  • [39]De Pina K, Navarro C, Mcwalter L, Boxer DH, Price NC, Kelly SM, Mandrand-Berthelot M-A, Wu L-F: Purification and characterization of the periplasmic nickel-binding protein NikA of escherichia coli K12. Eur J Biochem 1995, 227:857-865.
  • [40]Chung CT, Niemela SL, Miller RH: One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci U S A 1989, 86:2172-2175.
  • [41]Help: Assembly - parts.igem.org [http://parts.igem.org/Help:Assembly?title=Help:Assembly webcite]
  • [42]RBS [http://2009.igem.org/Team:Paris/Parts_RBS webcite]
  • [43]Part: BBa B0014 - parts.igem.org [http://parts.igem.org/Part:BBa_B0014 webcite]
  • [44]The Optimus [http://gcat.davidson.edu/igem10/opt/opt_index.html webcite]
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