NF-κB signaling and vesicle transport are correlated with the reactivation of the memory trace of morphine dependence

Diagnostic Pathology

Yun Liu¹  Ting Wang²  Yiming Wei⁵  Qin Zhang⁴  Daizhan Zhou⁶  Meimei Cai⁴  Candong Li⁴  Zhaoyang Yang⁴  Junyi Ye³ 

[1] Key Laboratory of Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, 303 Mingdao Building, 138 Yixueyuan Road, Shanghai 200032, PR China;Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, PR China;Institute of Biomedical Sciences, Fudan University, Shanghai, PR China;Fujian University of Traditional Chinese Medicine, Fuzhou, PR China;Fujian Medical University, Fuzhou, PR China;Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, PR China

关键词: NF-κB;    Methylome;    Transcriptome;    Morphine;    CPP;   

Others  :  870269
DOI  :  10.1186/1746-1596-9-142

 received in 2014-06-18, accepted in 2014-07-01,  发布年份 2014

【 摘 要 】

Background

Morphine has been widely used as a clinical anesthetic and analgesic. However, abuse of morphine might result in psychological and physiological dependence. Previous studies have indicated that memory mechanisms play critical roles in morphine dependence.

Methods

Morphine dependence was established in mice utilizing place preference conditioning (CPP). We observed changes in the methylome and transcriptome of the nucleus accumbens during the reactivation of the memory trace. We also monitored for changes in the methylome and transcriptome of mice that were acutely exposed to morphine.

Results

We detected 165 and 18 differentially expressed genes (DEGs) and 6 and 24 significant methyl-sensitive cut counting (MSCC) windows in the acute morphine treatment and the CPP model, respectively. The changes in the methylome and transcriptome during the acute treatment were mainly caused by a response to the morphine stimulus; most of the DEGs were correlated with hormone or transcription factor activity regulation. The expression levels of Lcn2 and Hspb1, which participate in the activation of NF-κB, were significantly decreased in the CPP morphine treatment model. Besides, the alternative splicing of the curtailed isoform of Caps1 was significantly increased in the CPP morphine-treated group, and the methylation levels of Arf4, Vapa, and Gga3 were decreased. These genes play critical roles in the regulation of the Golgi network.

Conclusions

The current study indicates that NF-κB signaling and vesicular transport are correlated with the reactivation of the memory trace in morphine-dependent mice. The results obtained in our study agree with previous observations and identify additional candidate genes for further research.

【 授权许可】

   
2014 Ye et al.; licensee BioMed Central Ltd.

【 图 表 】

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