Mature oligodendrocytes actively increase in vivo cytoskeletal plasticity following CNS damage

Journal of Neuroinflammation

Thorsten Buch¹  Burkhard Becher³  Ari Waisman²  Pushpalatha Palle¹  Bettina Schreiner³  Arianna Baggiolini³  Giuseppe Locatelli⁴ 

[1] Institute of Laboratory Animal Science, VetSuisse, University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland;Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg, University of Mainz, Obere Zahlbacher Str. 67, Mainz 55131, Germany;Institute of Experimental Immunology, University of Zurich, Winterthurerstrasse 190, Zürich 8057, Switzerland;Institute of Clinical Neuroimmunology, LMU Universität München, Marchioninistrasse 17, Munich 81377, Germany

关键词: Cytoskeletal dynamics;    CNS plasticity;    In vivo imaging;    Experimental autoimmune encephalomyelitis;    Demyelination;   

Others  :  1227103
DOI  :  10.1186/s12974-015-0271-2

 received in 2014-10-07, accepted in 2015-02-20,  发布年份 2015

【 摘 要 】

Background

Oligodendrocytes are myelinating cells of the central nervous system which support functionally, structurally, and metabolically neurons. Mature oligodendrocytes are generally believed to be mere targets of destruction in the context of neuroinflammation and tissue damage, but their real degree of in vivo plasticity has become a matter of debate. We thus investigated the in vivo dynamic, actin-related response of these cells under different kinds of demyelinating stress.

Methods

We used a novel mouse model (oLucR) expressing luciferase in myelin oligodendrocyte glycoprotein-positive oligodendrocytes under the control of a β-actin promoter. Activity of this promoter served as surrogate for dynamics of the cytoskeleton gene transcription through recording of in vivo bioluminescence following diphtheria toxin-induced oligodendrocyte death and autoimmune demyelination. Cytoskeletal gene expression was quantified from mature oligodendrocytes directly isolated from transgenic animals through cell sorting.

Results

Experimental demyelinating setups augmented oligodendrocyte-specific in vivo bioluminescence. These changes in luciferase signal were confirmed by further ex vivo analysis of the central nervous system tissue from oLucR mice. Increase in bioluminescence upon autoimmune inflammation was parallel to an oligodendrocyte-specific increased transcription of β-tubulin.

Conclusions

Mature oligodendrocytes acutely increase their cytoskeletal plasticity in vivo during demyelination. They are therefore not passive players under demyelinating conditions but can rather react dynamically to external insults.

【 授权许可】

   
2015 Locatelli et al.; licensee BioMed Central.

【 图 表 】

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