Journal of Nanobiotechnology | |
Development of antibody functionalized magnetic nanoparticles for the immunoassay of carcinoembryonic antigen: a feasibility study for clinical use | |
Kai-Wen Huang2  Bing-Hsien Liu1  Jui-Feng Chang1  Chia-Shin Ho1  Shieh-Yueh Yang1  Che-Chuan Yang1  | |
[1]MagQu Co., Ltd., Sindian Dist, New Taipei City 231, Taiwan | |
[2]Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan | |
关键词: Colorectal cancer; Assay; Carcinoembryonic antigen; Bio-magnetic nanoparticles; | |
Others : 1139420 DOI : 10.1186/s12951-014-0044-6 |
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received in 2014-08-10, accepted in 2014-10-13, 发布年份 2014 | |
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【 摘 要 】
Background
Magnetic nanoparticles functionalized antibodies are used for in-vitro assays on bio-markers. This work demonstrates the synthesis of high-quality magnetic nanoparticles coated with antibodies against carcinoembryonic antigen (CEA). Various characterizations, such as particle size, particle suspension, bio-activity and the stability of bio-magnetic nanoparticles suspended in liquid, are studied. The properties for the assay of CEA molecules in serum are also studied. The assay method used is so-called immunomagnetic reduction.
Results
The results show that the effects of common materials in serum that interfere with detected signals are not significant. The low-detection limit is 0.21 ng/ml, which is well below the clinical threshold of 2.5 ng/ml.
Conclusions
The dynamic range for the assay of CEA molecules in serum is 500 ng/ml. By assaying serum CEA molecules from 24 normal controls and 30 colorectal-cancer patients, the threshold for the serum-CEA concentration to diagnose colorectal cancer is 4.05 ng/ml, which results in a clinical sensitivity of 0.90 and specificity of 0.87.
【 授权许可】
2014 Yang et al.; licensee BioMed Central Ltd.
【 预 览 】
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