Development of antibody functionalized magnetic nanoparticles for the immunoassay of carcinoembryonic antigen: a feasibility study for clinical use

Journal of Nanobiotechnology

Methodology

Kai-Wen Huang¹  Bing-Hsien Liu²  Chia-Shin Ho²  Jui-Feng Chang²  Shieh-Yueh Yang²  Che-Chuan Yang² 

[1]Department of Surgery & Hepatitis Research Center, National Taiwan University Hospital, 100, Taipei, Taiwan

[2]Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, 100, Taipei, Taiwan

[3]MagQu Co., Ltd., Sindian Dist, 231, New Taipei City, Taiwan

关键词: Bio-magnetic nanoparticles;    Carcinoembryonic antigen;    Assay;    Colorectal cancer;   

DOI  :  10.1186/s12951-014-0044-6

 received in 2014-08-10, accepted in 2014-10-13,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundMagnetic nanoparticles functionalized antibodies are used for in-vitro assays on bio-markers. This work demonstrates the synthesis of high-quality magnetic nanoparticles coated with antibodies against carcinoembryonic antigen (CEA). Various characterizations, such as particle size, particle suspension, bio-activity and the stability of bio-magnetic nanoparticles suspended in liquid, are studied. The properties for the assay of CEA molecules in serum are also studied. The assay method used is so-called immunomagnetic reduction.ResultsThe results show that the effects of common materials in serum that interfere with detected signals are not significant. The low-detection limit is 0.21 ng/ml, which is well below the clinical threshold of 2.5 ng/ml.ConclusionsThe dynamic range for the assay of CEA molecules in serum is 500 ng/ml. By assaying serum CEA molecules from 24 normal controls and 30 colorectal-cancer patients, the threshold for the serum-CEA concentration to diagnose colorectal cancer is 4.05 ng/ml, which results in a clinical sensitivity of 0.90 and specificity of 0.87.

【 授权许可】

CC BY   
© Yang et al.; licensee BioMed Central Ltd. 2014