Therapeutic Electromagnetic Field (TEMF) and gamma irradiation on human breast cancer xenograft growth, angiogenesis and metastasis

Cancer Cell International

C Douglas Williams³  W Elaine Hardman¹  Nicholas Short²  Lu-Zhe Sun²  Ivan L Cameron² 

[1] Pennington Biomedical Research Center, Louisiana State University, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USA;Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78229, USA;EMF Therapeutics, Inc., P.O. Box 679, Signal Mountain, Tennessee 37377, USA

关键词: metastasis;    angiogenesis;    ionizing irradiation;    breast cancer;    electromagnetic field;   

Others  :  795979
DOI  :  10.1186/1475-2867-5-23

 received in 2004-07-05, accepted in 2005-07-26,  发布年份 2005

【 摘 要 】

Background

The effects of a rectified semi-sinewave signal (15 mT amplitude, 120 pulses per second, EMF Therapeutics, Inc.) (TEMF) alone and in combination with gamma irradiation (IR) therapy in nude mice bearing a human MDA MB231 breast cancer xenograft were tested. Green fluorescence protein transfected cancer cells were injected into the mammary fat pad of young female mice. Six weeks later, mice were randomly divided into four treatment groups: untreated controls; 10 minute daily TEMF; 200 cGy of IR every other day (total 800 cGy); IR plus daily TEMF. Some mice in each group were euthanized 24 hours after the end of IR. TEMF treatment continued for 3 additional weeks. Tumor sections were stained for: endothelial cells with CD31 and PAS or hypoxia inducible factor 1α (HIF).

Results

Most tumors <35 mm³ were white but tumors >35 mm³ were pink and had a vascularized capsule. The cortex within 100 microns of the capsule had little vascularization. Blood vessels, capillaries, and endothelial pseudopods were found at >100 microns from the capsule (subcortex). Tumors >35 mm³ treated with IR 24 hours previously or with TEMF had decreased blood vessels in the subcortex and more endothelial pseudopods projecting into hypoxic, HIF positive areas than tumors from the control group. Mice that received either IR or TEMF had significantly fewer lung metastatic sites and slower tumor growth than did untreated mice. No harmful side effects were attributed to TEMF.

Conclusion

TEMF therapy provided a safe means for retarding tumor vascularization, growth and metastasis.

【 授权许可】

   
2005 Cameron et al; licensee BioMed Central Ltd.

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