Genomic characterization of a Helicobacter pylori isolate from a patient with gastric cancer in China

Gut Pathogens

Jianzhong Zhang²  Dongfang Li¹  Lihua He²  Yuanfang Zhu¹  Maojun Zhang²  Lin Liu¹  Yuanhai You² 

[1] BGI-Shenzhen, Shenzhen, People’s Republic of China;Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Infectious Disease Prevention and Control and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China

关键词: Genomic features;    Next generation sequencing;    Gastric cancer;    Helicobacter pylori;   

Others  :  817560
DOI  :  10.1186/1757-4749-6-5

 received in 2014-01-02, accepted in 2014-02-18,  发布年份 2014

【 摘 要 】

Background

Helicobacter pylori is well known for its relationship with the occurrence of several severe gastric diseases. The mechanisms of pathogenesis triggered by H. pylori are less well known. In this study, we report the genome sequence and genomic characterizations of H. pylori strain HLJ039 that was isolated from a patient with gastric cancer in the Chinese province of Heilongjiang, where there is a high incidence of gastric cancer. To investigate potential genomic features that may be involved in pathogenesis of carcinoma, the genome was compared to three previously sequenced genomes in this area.

Result

We obtained 42 contigs with a total length of 1,611,192 bp and predicted 1,687 coding sequences. Compared to strains isolated from gastritis and ulcers in this area, 10 different regions were identified as being unique for HLJ039; they mainly encoded type II restriction-modification enzyme, type II m6A methylase, DNA-cytosine methyltransferase, DNA methylase, and hypothetical proteins. A unique 547-bp fragment sharing 93% identity with a hypothetical protein of Helicobacter cinaedi ATCC BAA-847 was not present in any other previous H. pylori strains. Phylogenetic analysis based on core genome single nucleotide polymorphisms shows that HLJ039 is defined as hspEAsia subgroup, which belongs to the hpEastAsia group.

Conclusion

DNA methylations, variations of the genomic regions involved in restriction and modification systems, are the “hot” regions that may be related to the mechanism of H. pylori-induced gastric cancer. The genome sequence will provide useful information for the deep mining of potential mechanisms related to East Asian gastric cancer.

【 授权许可】

   
2014 You et al.; licensee BioMed Central Ltd.

【 图 表 】

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