Higher frequency of cagA EPIYA-C Phosphorylation Sites in H. pylori strains from first-degree relatives of gastric cancer patients

BMC Gastroenterology

Lucia LBC Braga²  Aldo AM Lima³  Richard L Guerrant¹  Sérgio A Batista⁴  Andreia MC Rocha⁴  Gifone A Rocha⁴  André MN Fialho⁴  Andréa BC Fialho²  Manuel B Braga-Neto²  Maria HRB Goncalves²  Cícero ISM Silva²  Dulciene MM Queiroz⁴ 

[1] Center for Global Health, University of Virginia, Charlottesville, VA, USA;Clinical Research Unity – Department of Internal Medicine, University Hospital Walter Cantídio – Federal University of Ceará, P.O. Box: 60430270, Fortaleza, Ceará, Brazil;Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil;Laboratory of Research in Bacteriology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

关键词: H. pylori/vacA;    H. pylori CagA-EPIYA;    Gastric cancer;    Helicobacter pylori;   

Others  :  1121892
DOI  :  10.1186/1471-230X-12-107

 received in 2012-02-29, accepted in 2012-08-08,  发布年份 2012

【 摘 要 】

Background

To evaluate the prevalence of more virulent H. pylori genotypes in relatives of gastric cancer patients and in patients without family histories of gastric cancer.

Methods

We evaluated prospectively the prevalence of the infection by more virulent H. pylori strains in 60 relatives of gastric cancer patients comparing the results with those obtained from 49 patients without family histories of gastric cancer. H. pylori status was determined by the urease test, histology and presence of H. pylori ureA. The cytotoxin associated gene (cagA), the cagA-EPIYA and vacuolating cytotoxin gene (vacA) were typed by PCR and the cagA EPIYA typing was confirmed by sequencing.

Results

The gastric cancer relatives were significant and independently more frequently colonized by H. pylori strains with higher numbers of CagA-EPIYA-C segments (OR = 4.23, 95%CI = 1.53–11.69) and with the most virulent s1m1 vacA genotype (OR = 2.80, 95%CI = 1.04–7.51). Higher numbers of EPIYA-C segments were associated with increased gastric corpus inflammation, foveolar hyperplasia and atrophy. Infection by s1m1 vacA genotype was associated with increased antral and corpus gastritis.

Conclusions

We demonstrated that relatives of gastric cancer patients are more frequently colonized by the most virulent H. pylori cagA and vacA genotypes, which may contribute to increase the risk of gastric cancer.

【 授权许可】

   
2012 Queiroz et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1 .

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