Females transplanted with ovaries subjected to hypoxic preconditioning show impair of ovarian function

Journal of Ovarian Research

Edmund Chada Baracat⁴  José Eduardo Krieger¹  Edna Frasson de Souza Montero³  Ricardo dos Santos Simões⁴  Gustavo Arantes Rosa Maciel⁴  José Maria Soares-Jr⁴  Juliana Sanajotti Nakamuta¹  Luciana Lamarão Damous² 

[1] Laboratory of Genetics and Molecular Cardiology, Heart Institute (Incor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil;Galvão Bueno St, 499. Bloco A. Apto31. Liberdade, São Paulo 01506-000, SP, Brazil;Department of Surgery, Laboratory of Surgical Physiopathology (LIM-62), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil;Gynecology Division, Department of Obstetrics and Gynecology, Laboratory of Structural and Molecular Gynecology (LIM-58), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil

关键词: Rat;    Ovary;    Apoptosis;    Hypoxic preconditioning;    Ovarian transplantation;   

Others  :  801738
DOI  :  10.1186/1757-2215-7-34

 received in 2014-01-20, accepted in 2014-03-10,  发布年份 2014

【 摘 要 】

Background

Cryopreservation of the ovarian tissue has shown promising results. However, there remain controversial issues such as the short half-life of grafts. In this aspect, there are some evidences that preconditioning the ovarian tissue before transplantation is beneficial.

Objective

To determine the effect of hypoxic preconditioning in vitro on ovarian tissue prior to transplantation.

Methods

Eighteen female adult Wistar rats, were sorted into three experimental groups. Ovaries were maintained in DMEM low glucose serum free at 37°C with 5% CO2, at atmospheric oxigen concentration (normoxia) or 1% O₂ (hypoxia) for 16 hours. Oxigen concentration was determined by injection of nitrogen in the incubator. Animals submitted to ovarian transplantation immediately after oophorectomy were the Control Group (C). After this, the ovaries were implanted in the retroperitoneum with nonabsorbable suture and animals evaluated for thirty days after transplantation. Beginning on postoperative (PO) day 11, a daily collection of vaginal smear was carried out. Analyses comprised morphological, morphometric (counting ovarian follicles and corpora lutea) and immunohistochemistry for cleaved caspase-3 (apoptosis).

Results

In normoxia and control groups all animals recovered their estrous cycles, while in the hypoxia group, two animals did not ovulate but, among those which did, resumption took longer than in the other groups (p < 0.05). The number of ovarian follicles and corpora lutea decreased significantly in the hypoxia group when compared to the other two groups (p < 0.001) and apoptosis was increased in the few ovarian follicles which remained viable (p < 0.001).

Conclusion

The hypoxic preconditioning in vitro was not beneficial to the graft and worsened their viability, compromising its functionality or delaying the return of this.

【 授权许可】

   
2014 Damous et al.; licensee BioMed Central Ltd.

【 图 表 】

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