BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause

BMC Cell Biology

Jian-ming Tan¹  Feng Zheng¹  Lian-ming Liao¹  Shun-liang Yang¹  Fu-qiang He¹  Zhi-xian Wu¹  Xiao-wen Chen¹  Yu-jie Ma¹  Jin Wang¹  Yun-fen Fu¹  Jun Lu¹  Jin Chen¹  Hui-yue Dong¹  Liang-hu Huang¹  Wei-zhen Wu¹  Zhi-jie Lin¹  Xia Gao¹  Jun-qi Guo¹ 

[1] Organ Transplant Institute, Fuzhou General Hospital, DongFang Hospital of Xiamen University and Fujian Key Laboratory of Transplant Biology, Fujian, 350025, PR. China

关键词: Perimenopause;    Rat;    Ovary;    Apoptosis;    Granulosa cells;    Bone marrow mesenchymal stem cells;   

Others  :  855391
DOI  :  10.1186/1471-2121-14-18

 received in 2012-10-15, accepted in 2013-02-20,  发布年份 2013

【 摘 要 】

Background

The objective of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BMSCs) on the apoptosis of granulosa cells (GCs) in rats.

BMSCs and GCs were isolated from rats. GCs were separated into one of the following three groups: an untreated control group (control), a cisplatin (5 mg/L) treatment group (cisplatin), and group co-cultured with BMSCs and treated with cisplatin (BMSC). GC apoptosis was analyzed by annexin V staining and real-time PCR analysis for apoptosis-related genes. The effect of BMSCs was also determined in 9 to 10 month-old perimenopausal rats that were separated into the following groups: saline control, BMSC transplantation (1–2 × 10⁶ cells), and estrogen treatment (0.158 mg/kg/d) groups. A young group consisting of 3 to 4 month-old rats that were treated with saline was also evaluated as a control. After 1 and 3 months, GC apoptosis was evaluated by TUNEL analysis.

Results

Cisplatin increased GC apoptosis from 0.59% to 13.04% in the control and cisplatin treatment groups, respectively, which was significantly reduced upon co-culture with BMSCs to 4.84%. Cisplatin treatment increased p21 and bax and decreased c-myc mRNA expression, which was reversed upon co-culture with BMSCs. As compared to young rats, increased apoptosis was observed in the perimenopausal rats (P < 0.001). After 3 months, the apoptosis rate in the BMSC group was significantly lower than that of the control group (P = 0.007).

Conclusions

BMSC therapy may protect against GC apoptosis induced by cisplatin and perimenopause. Further studies are necessary to evaluate therapeutic efficacy of BMSCs.

【 授权许可】

   
2013 Guo et al.; licensee BioMed Central Ltd.

【 图 表 】

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