Early-age feed restriction affects viability and gene expression of satellite cells isolated from the gastrocnemius muscle of broiler chicks

Journal of Animal Science and Biotechnology

Ruqian Zhao²  Roland Grossmann³  Nadia Everaert¹  Johan Buyse¹  Eddy Decuypere¹  Yingdong Ni²  Xiaojing Yang²  Yue Li¹ 

[1]Laboratory of Livestock Physiology, Immunology and Genetics, K.U. Leuven, Leuven, Belgium

[2]Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing 210095, China

[3]Department of Functional Genomics & Bioregulation, Institute of Animal Genetics Mariensee, FLI, 31535, Neustadt, Germany

关键词: Chicken;    Gene expression;    Proliferation;    Satellite cells;    Feed restriction;   

Others  :  813378
DOI  :  10.1186/2049-1891-3-33

 received in 2012-05-15, accepted in 2012-11-01,  发布年份 2012

【 摘 要 】

Background

Muscle growth depends on the fusion of proliferate satellite cells to existing myofibers. We reported previously that 0–14 day intermittent feeding led to persistent retardation in myofiber hypertrophy. However, how satellite cells respond to such nutritional insult has not been adequately elucidated.

Results

One-day-old broiler chicks were allocated to control (Con, ad libitum feeding), intermittent feeding (IF, feed provided on alternate days) and re-feeding (RF, 2 days ad libitum feeding after 12 days of intermittent feeding) groups. Chickens were killed on Day 15 and satellite cells were isolated. When cultured, satellite cells from the IF group demonstrated significant retardation in proliferation and differentiation potential, while RF partly restored the proliferation rate and differentiation potential of the satellite cells. Significant up-regulation of insulin like growth factor I receptor (IGF-IR) (P<0.05) and thyroid hormone receptor α (TRα) (P<0.05), and down-regulation of growth hormone receptor (GHR) (P<0.01) and IGF-I (P<0.01) mRNA expression was observed in freshly isolated IF satellite cells when compared with Con cells. In RF cells, the mRNA expression of IGF-I was higher (P<0.05) and of TRα was lower (P<0.01) than in IF cells, suggesting that RF restored the mRNA expression of TRα and IGF-I, but not of GHR and IGF-IR. The Bax/Bcl-2 ratio tended to increase in the IF group, which was reversed in the RF group (P<0.05), indicating that RF reduced the pro-apoptotic influence of IF. Moreover, no significant effect of T₃ was detected on cell survival in IF cells compared with Con (P<0.001) or RF (P<0.05) cells.

Conclusions

These data suggest that early-age feed restriction inhibits the proliferation and differentiation of satellite cells, induces changes in mRNA expression of the GH/IGF-I and thyroid hormone receptors in satellite cells, as well as blunted sensitivity of satellite cells to T₃, and that RF partially reverses these effects. Thus, a moderate nutritional strategy for feed restriction should be chosen in early chick rearing systems.

【 授权许可】

   
2012 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

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