Effects of extremely low frequency electromagnetic fields on paraoxonase serum activity and lipid peroxidation metabolites in rat

Journal of Diabetes & Metabolic Disorders

Bagher Larijani³  Maryam Razzaghy-Azar¹  Mahsa Mohammad Amoli³  Mitra Nourbakhsh¹  Shahrokh Farzampour²  Soroush Seifirad³ 

[1] Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran;Electromagnetic Waves Research Center, Artesh University of Medical Sciences, Tehran, Iran;Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

关键词: Free fatty acids;    Magnetic fields;    Electromagnetic radiation;    Lipid peroxidation;    Paraoxonase;   

Others  :  1136146
DOI  :  10.1186/s40200-014-0085-2

 received in 2014-06-21, accepted in 2014-08-03,  发布年份 2014

【 摘 要 】

Background

Atherogenic effects of ELF-MF exposure have not been studied well so far. Therefore we have hypothesized that ELF-MF exposure might have atherogenic effect by impairing antioxidant function and increasing lipid peroxidation. This study was therefore undertaken to examine the effects of ELF-MF on paraoxonase (PON) activity, antioxidant capacity and lipid peroxidation metabolites. Effects of time on remodeling of antioxidant system were also investigated in this study.

Methods

Seventy five Wistar rats were randomly allocated into five groups as follows: 1) Sham exposure, 2) Single exposure to 60 Hz, sacrificed immediately after exposure, 3) Single exposure to 60 Hz, sacrificed 72 hours after exposure, 4) Fourteen days of exposure to 60 Hz, sacrificed immediately after exposure, and 5) Fourteen days of exposure to 60 Hz, sacrificed 72 hours after exposure. Blood samples were collected and analyzed. The results were compared using ANOVA and post hoc Tukey HSD for multiple caparisons.

Results

Single ELF-MF exposure significantly increased lipid peroxidation (CD and MDA) and increased antioxidant serum activity (HDL, paraoxonase activity, and serum total antioxidant capacity). Chronic ELF-MF exposure increased lipid peroxidation and affected antioxidant system. Free fatty acids levels were significantly increased after both single and two weeks exposure. Chronic exposure led to irreversible changes while acute exposure tended to reversible alterations on above mentioned parameters.

Conclusions

According to the results of this study, ELF-MF exposure could impair oxidant-antioxidant function and might increase oxidative stress and lipid peroxidation. Antioxidant capability was dependent on the duration and continuity of ELF-MF exposure.

【 授权许可】

   
2014 Seifirad et al.; licensee BioMed Central Ltd.

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