Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue

Breast Cancer Research

Lyn R Griffiths¹  Stephen R Weinstein²  Joanne E Curran¹  Rod A Lea¹  Robert A Smith¹ 

[1] Genomics Research Centre and Wesley Research Institute, Griffith University Gold Coast, Queensland, Australia;Department of Pathology, Gold Coast Hospital, Southport, Queensland, Australia

关键词: progesterone receptor;    nuclear receptors;    glucocorticoid receptor;    breast cancer;   

Others  :  1118816
DOI  :  10.1186/bcr556

 received in 2002-06-10, accepted in 2002-10-21,  发布年份 2002

【 摘 要 】

Background

Previous studies in our laboratory have shown associations of specific nuclear receptor gene variants with sporadic breast cancer. In order to investigate these findings further, we conducted the present study to determine whether expression levels of the progesterone and glucocorticoid nuclear receptor genes vary in different breast cancer grades.

Methods

RNA was extracted from paraffin-embedded archival breast tumour tissue and converted into cDNA. Sample cDNA underwent PCR using labelled primers to enable quantitation of mRNA expression. Expression data were normalized against the 18S ribosomal gene multiplex and analyzed using analysis of variance.

Results

Analysis of variance indicated a variable level of expression of both genes with regard to breast cancer grade (P = 0.00033 for glucocorticoid receptor and P = 0.023 for progesterone receptor).

Conclusion

Statistical analysis indicated that expression of the progesterone nuclear receptor is elevated in late grade breast cancer tissue.

【 授权许可】

   
2003 Smith et al., licensee BioMed Central Ltd

【 参考文献 】

• [1]King M-C, Roswell S, Love SM: Inherited breast and ovarian cancer: what are the risks? What are the choices? JAMA 1993, 269:1975-1980.
• [2]Zheng L, Li S, Boyer TG, Lee WH: Lessons learned from BRCA1 and BRCA2. Oncogene 2000, 19:6159-6175.
• [3]Curran JE, Weinstein SR, Griffiths LR: Polymorphisms of glutathione S-transferase genes (GSTM1, GSTP1 and GSTT1) and breast cancer susceptibility. Cancer Lett 2000, 153:113-120.
• [4]Berardo D, Allred DC, O'Connell P: Breast cancer. In Principles of molecular medicine. Edited by Totowa NJ. Humana Press; 1998:625-632.
• [5]Fryer CJ, Kinyamu HK, Rogatsky I, Garabedian MJ, Archer TK: Selective activation of the glucocorticoid receptor by steroid antagonists in human breast cancer and osteosarcoma cells. J Biol Chem 2000, 275:17771-17777.
• [6]Keightley M-C: Steroid receptor isoforms: exception or rule? Mol Cell Endocrinol 1998, 137:1-5.
• [7]Dickson RB, Lippman ME: Control of human breast cancer by estrogen, growth factors, and oncogenes. Cancer Treat Res 1988, 40:119-165.
• [8]Lee Y-N, Lee H-Y, Lee YM, Chung H-Y, Kim S-I, Lee S-K, Park BC, Kim K-W: Involvement of glucocorticoid receptor in the induction of differentiation by ginsenosides in F9 teratocarcinoma cells. J Steroid Biochem Mol Biol 1998, 67:105-111.
• [9]Hulkko SM, Zilliacus J: Functional interaction between the pro-apoptotic DAP3 and the glucocorticoid receptor. Biochem Biophys Res Commun 2002, 295:749-755.
• [10]Curran JE, Vaughan T, Lea RA, Weinstein SR, Morrison NA, Griffiths LR: Association of a vitamin D receptor polymorphism with sporadic breast cancer development. Int J Cancer 1999, 83:723-726.
• [11]Karck U, Kommoss F: Does tamoxifen change oestrogen and progesterone receptor expression in the endometrium and breast? Eur J Cancer 2000, 36:S39-S46.
• [12]Selvey S, Thompson EW, Matthaei K, Lea RA, Irving MG, Griffiths LR: B-Actin: an unsuitable internal control for RT-PCR. Mol Cell Probes 2001, 15:307-311.
• [13]Dunn OJ: Multiple contrasts using rank sums. Technometrics 1964, 6:241-252.
• [14]Fazzari A, Catalano MG, Comba A, Becchis M, Raineri M, Frairia R, Fortunati N: The control of progesterone receptor expression in MCF-7 breast cancer cells: effects of estradiol and sex hormone-binding globulin (SHBG). Mol Cell Endocrinol 2001, 172:31-36.
• [15]Krishnan AV, Swami S, Feldman D: Estradiol inhibits glucocorticoid receptor expression and induces glucocorticoid resistance in MCF-7 human breast cancer cells. J Steroid Biochem Mol Biol 2001, 77:29-37.
• [16]Pieber D, Allport VC, Bennett PR: Progesterone receptor isoform A inhibits isoform B-mediated transactivation in human amnion. Eur J Pharmacol 2001, 427:7-11.