In vitro assessment of antibody-conjugated gold nanorods for systemic injections

Journal of Nanobiotechnology

Roberto Pini³  Franco Fusi⁵  Enrico Mini²  Giovanna Marrazza¹  Andrea Ravalli¹  Stefania Nobili⁴  Ida Landini⁴  Giovanni Romano⁵  Raffaella Mercatelli¹  Alessio Gnerucci⁵  Fulvio Ratto³  Francesca Tatini³  Sonia Centi⁵ 

[1] Dipartimento di Chimica ‘Ugo Shiff’, Università degli Studi di Firenze, Via della Lastruccia 3, Sesto Fiorentino, 50019, Italy;Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Firenze, Largo Brambilla 3, Firenze, 50134, Italy;Istituto di Fisica Applicata ‘Nello Carrara’, Consiglio Nazionale delle Ricerche, Via Madonna del Piano 10, Sesto Fiorentino, 50019, Italy;Dipartimento di Scienze della Salute, Università degli Studi di Firenze, Viale Pieraccini 6, Firenze, 50139, Italy;Dipartimento di Scienze Biomediche Sperimentali e Cliniche ‘Mario Serio’, Università degli Studi di Firenze, Viale Pieraccini 6, Firenze, 50139, Italy

关键词: Blood compatibility;    Matrix effect;    Competitive assay;    Active targeting;    Cancer antigen 125;    Gold nanorods;   

Others  :  1139299
DOI  :  10.1186/s12951-014-0055-3

 received in 2014-09-10, accepted in 2014-11-22,  发布年份 2014

【 摘 要 】

Background

The interest for gold nanorods in biomedical optics is driven by their intense absorbance of near infrared light, their biocompatibility and their potential to reach tumors after systemic administration. Examples of applications include the photoacoustic imaging and the photothermal ablation of cancer. In spite of great current efforts, the selective delivery of gold nanorods to tumors through the bloodstream remains a formidable challenge. Their bio-conjugation with targeting units, and in particular with antibodies, is perceived as a hopeful solution, but the complexity of living organisms complicates the identification of possible obstacles along the way to tumors.

Results

Here, we present a new model of gold nanorods conjugated with anti-cancer antigen 125 (CA125) antibodies, which exhibit high specificity for ovarian cancer cells. We implement a battery of tests in vitro, in order to simulate major nuisances and predict the feasibility of these particles for intravenous injections. We show that parameters like the competition of free CA125 in the bloodstream, which could saturate the probe before arriving at the tumors, the matrix effect and the interference with erythrocytes and phagocytes are uncritical.

Conclusions

Although some deterioration is detectable, anti-CA125-conjugated gold nanorods retain their functional features after interaction with blood tissue and so represent a powerful candidate to hit ovarian cancer cells.

【 授权许可】

   
2014 Centi et al.; licensee BioMed Central Ltd.

【 图 表 】

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