Bone marrow T cells from the femur are similar to iliac crest derived cells in old age and represent a useful tool for studying the aged immune system

Immunity & Ageing

Beatrix Grubeck-Loebenstein³  Martin Krismer¹  Birgit Weinberger³  Robert Gassner⁴  Julian Lair¹  Rauend Rauf¹  Dietmar Herndler-Brandstetter²  Katja Landgraf-Rauf³  Theresa Pritz³ 

[1] Department of Orthopedic Surgery, Innsbruck Medical University, Anichstrasse 35, Innsbruck, Austria;Present address: Yale University School of Medicine, 333 Cedar Street, New Haven, CT, USA;Institute for Biomedical Aging Research, University Innsbruck, Rennweg 10, Innsbruck, Austria;Department of Cranio-Maxillofacial and Oral Surgery, Innsbruck Medical University, Anichstrasse 35, Innsbruck, Austria

关键词: Cytomegalovirus;    T cell lines;    Cytokines;    T cells;    Mononuclear cells;    Peripheral blood;    Femur;    Iliac crest;    Bone marrow;   

Others  :  815034
DOI  :  10.1186/1742-4933-10-17

 received in 2012-12-21, accepted in 2013-03-25,  发布年份 2013

【 摘 要 】

Background

CD4⁺ and CD8⁺ T cells reside in the human bone marrow (BM) and show a heightened activation state. However, only small sample sizes are available from sources such as the iliac crest. Larger samples can be obtained from the femur in the course of hip replacement surgery. It was therefore the goal of the present study to compare the phenotype and function of BM T cells from different sources from elderly persons and to investigate how femur derived bone marrow T cells can serve as a tool to gain a better understanding of the role of adaptive immune cells in the BM in old age.

Results

Bone marrow mononuclear cells (BMMC) were isolated from either the iliac crest or the femur shaft. As expected the yield of mononuclear cells was higher from femur than from iliac crest samples. There were no phenotypic differences between BMMC from the two sources. Compared to PBMC, both BM sample types contained fewer naïve and more antigen experienced CD4⁺ as well as CD8⁺ T cells, which, in contrast to peripheral cells, expressed CD69. Cytokine production was also similar in T cells from both BM types. Larger sample sizes allowed the generation of T cell lines from femur derived bone marrow using non-specific as well as specific stimulation. The phenotype of T cell lines generated by stimulation with OKT-3 and IL-2 for two weeks was very similar to the one of ex vivo BM derived T cells. Such lines can be used for studies on the interaction of different types of BM cells as shown by co-culture experiments with BM derived stromal cells. Using CMV_NLV specific T cell lines we additionally demonstrated that BM samples from the femur are suitable for the generation of antigen specific T cell lines, which can be used in studies on the clonal composition of antigen specific BM T cells.

Conclusion

In conclusion, our results demonstrate that BMMC from the femur shaft are a useful tool for studies on the role of T cells in the BM in old age.

【 授权许可】

   
2013 Pritz et al.; licensee BioMed Central Ltd.

【 图 表 】

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