Vitamin d deficiency impacts on expression of toll-like receptor-2 and cytokine profile: a pilot study

Journal of Translational Medicine

Kumar Visvanathan³  Ian Woolley²  Vijaya Sundararajan²  Boyd JG Strauss²  Narelle A Skinner³  Samar Ojaimi¹ 

[1] Department of Infectious Diseases, Southern Health, Clayton Rd, Clayton, Victoria, Australia;Department of Medicine, Southern Clinical School, Monash University, Clayton Rd, Clayton, Victoria, Australia;Departments of Infectious Diseases and Medicine, Clinical Immunology Laboratory, St Vincent’s Hospital, University of Melbourne, 4th Floor, Clinical Sciences Building, Fitzroy, Victoria 3065, Australia

关键词: Innate immunity;    Immunity;    Toll-like receptor;    Vitamin D deficiency;    Vitamin D;   

Others  :  827066
DOI  :  10.1186/1479-5876-11-176

 received in 2013-03-12, accepted in 2013-07-01,  发布年份 2013

【 摘 要 】

Background

Vitamin D is believed to play an important role outside the endocrine system in the regulation of the immune system, and in cellular proliferation and differentiation. The aim of the study was to investigate the impact of vitamin D levels on innate immunity.

Methods

Participants for this prospective, longitudinal study were recruited amongst otherwise healthy staff of a large hospital in Victoria, Australia. Those fulfilling the inclusion criteria, including a vitamin D level of <50 nmol/L, were supplemented. Using flow cytometry, expression of the innate immune receptors TLR2, TLR4 and CD86 was measured on peripheral blood mononuclear cells (PBMCs) collected prior to vitamin D treatment and then at 1 and 3 months. Additonally, PBMCs at each timepoint were stimulated with specific TLR ligands and resultant supernatants were assayed for the cytokines TNFα, IL-6, IFN-α and IP-10.

Results

In participants whose vitamin D level was >100 nmol/L post supplementation (n=11), TLR2 expression on PBMCs increased significantly, with no change noted in TLR4 or CD86 expression. Stimulation of vitamin D deficient samples with TLR ligands produced a number of proinflammatory cytokines, which were significantly reduced upon vitamin D normalisation. In patients whose levels returned to a deficient level at 3 months despite ongoing low-level supplementation, an increase in the pro-inflamamtory state returned. This suggests that vitamin D may play an important role in ensuring an appropriate baseline pro-inflammatory state.

Conclusions

This ex-vivo pilot study adds clinical evidence supporting a possibly important role for vitamin D in innate immunity. If confirmed, this unique clinical study has potentially significant implications for the treatment of a variety of inflammatory conditions, where achieving optimal vitamin D levels may help reduce inflammation.

【 授权许可】

   
2013 Ojaimi et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 2.

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