Anti-inflammatory effects of Morus alba Linne bark on the activation of toll-like receptors and imiquimod-induced ear edema in mice

BMC Complementary Medicine and Therapies

Lin Umeyama¹  Shiori Kasahara¹  Yoshihiro Hayakawa¹  Dya Fita Dibwe¹  Suresh Awale¹  Satoru Yokoyama²  Besse Hardianti³ 

[1] Institute of Natural Medicine, University of Toyama, 2630 Sugitani, 930-0194, Toyama, Japan;Institute of Natural Medicine, University of Toyama, 2630 Sugitani, 930-0194, Toyama, Japan;Department of Cancer Cell Biology, Graduate School of Medicine and Pharmaceutical Science, University of Toyama, 2630 Sugitani, 930-0194, Toyama, Japan;Institute of Natural Medicine, University of Toyama, 2630 Sugitani, 930-0194, Toyama, Japan;Sekolah Tinggi Ilmu Farmasi Makassar, Perintis Kemerdekaan Street Km 13.7, 90242, Makassar, Indonesia;

关键词: Inflammation;    Toll-like receptor;    Psoriasis;    Innate immunity;    Morus alba;   

DOI  :  10.1186/s12906-021-03291-5

来源: Springer

【 摘 要 】

BackgroundMorus alba L. bark has been widely used in traditional medicine for treating several inflammatory diseases, such as hypertension, diabetes mellitus and coughing; however, the molecular mechanisms underlying its anti-inflammatory effects are not well understood.MethodsWe examined the effects of an extract of Morus alba L. bark (MabE) on Toll-like receptor (TLR) ligand-induced activation of RAW264.7 macrophages using a luciferase reporter assay and immunoassays. For the in vivo experiment, we used an imiquimod-induced ear edema model to examine the anti-inflammatory effects of MabE.ResultsMabE inhibited the TLR ligand-induced activation of NF-κB in RAW264.7 cells without affecting their viability. Consistent with the inhibition of NF-κB activation, MabE also inhibited the production of IL-6 and IL-1β from TLR ligand-treated RAW264.7 cells. In vivo MabE treatment inhibited the ear swelling of IMQ-treated mice, in addition to the mRNA expression of IL-17A, IL-1β and COX-2. The increases in splenic γδT cells in IMQ-treated mice and the production of IL-17A from splenocytes were significantly inhibited by MabE treatment.ConclusionOur study suggests that the anti-inflammatory effects of MabE on the activation of the macrophage cell line RAW246.7 by TLRs and IMQ-induced ear edema are through the inhibition of NF-κB activation and IL-17A-producing γδT cells, respectively.

【 授权许可】

CC BY