Anaplasma phagocytophilum strains from voles and shrews exhibit specific ankA gene sequences

BMC Veterinary Research

Friederike D von Loewenich³  Richard Birtles¹  Kathrin Hartelt²  Nicole Wüppenhorst³  Juliana Majazki³ 

[1] School of Environment and Life Sciences, University of Salford, The Crescent, Salford M5 4WT, UK;Baden-Wuerttemberg State Health Office, District Government Stuttgart, Nordbahnhofstrasse 135, Stuttgart D-70191, Germany;Institute of Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Strasse 11, Freiburg D-79104, Germany

关键词: Recombination;    ankA gene;    Genotyping;    Shrews;    Voles;    Anaplasma phagocytophilum;   

Others  :  1119403
DOI  :  10.1186/1746-6148-9-235

 received in 2013-05-10, accepted in 2013-11-25,  发布年份 2013

【 摘 要 】

Background

Anaplasma phagocytophilum is a Gram-negative bacterium that replicates obligate intracellularly in neutrophils. It is transmitted by Ixodes spp. ticks and causes acute febrile disease in humans, dogs, horses, cats, and livestock. Because A. phagocytophilum is not transmitted transovarially in Ixodes spp., it is thought to depend on reservoir hosts to complete its life cycle. In Europe, A. phagocytophilum was detected in roe deer, red deer, wild boars, and small mammals. In contrast to roe deer, red deer and wild boars have been considered as reservoir hosts for granulocytic anaplasmosis in humans, dogs, and horses according to groESL- and ankA-based genotyping. A. phagocytophilum variants infecting small mammals in Europe have not been characterized extensively to date.

Results

We amplified the total ankA open reading frames of 27 strains from voles and shrews. The analysis revealed that they harboured A. phagocytophilum strains that belonged to a distinct newly described ankA gene cluster. Further, we provide evidence that the heterogeneity of ankA gene sequences might have arisen via recombination.

Conclusions

Based on ankA-based genotyping voles and shrews are unlikely reservoir hosts for granulocytic anaplasmosis in humans, dogs, horses, and livestock in Europe.

【 授权许可】

   
2013 Majazki et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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