Mycobacterium leprae Hsp65 administration reduces the lifespan of aged high antibody producer mice

Immunity & Ageing

Osvaldo Augusto Sant’Anna⁴  Valquiria Bueno¹  Nancy Starobinas²  Marcelo de Franco²  Eliana Blini Marengo³  Estevam José Baldon⁴ 

[1] Departamento de Microbiologia, Imunologia e Parasitologia, UNIFESP, Rua Botucatu 862, 04023-062 São Paulo, Brasil;Laboratório de Imunogenética, Instituto Butantan, Avenida Vital Brazil 1500, 05530-900 São Paulo, Brasil;Hospital Israelita Albert Einstein, Avenida Albert Einstein, 627/701, 05652-000 São Paul, Brasil;Laboratório de Imunoquímica, Instituto Butantan, Avenida Vital Brazil 1500, 05530-900 São Paulo, Brasil

关键词: Antibody response;    Immunosenescence;    Aging;    Hsp65;    Heat shock protein;   

Others  :  814007
DOI  :  10.1186/1742-4933-11-6

 received in 2013-11-26, accepted in 2014-03-22,  发布年份 2014

【 摘 要 】

Background

Aging process may result in immune modifications that lead to disruption of innate and acquired immunity mechanisms that may induce chronic-degenerative events. The heat shock proteins (Hsp), phylogeneticaly conserved among organisms, present as main function the ability of folding and refolding proteins, but they also are associated with chronic-degenerative disorders. Here were evaluated the role of M. leprae native Hsp65 (WT) and its point-mutated (K⁴⁰⁹A) on survival and anti-DNA and anti-Hsp65 antibody production of aged genetically selected mice for high (H_III) and low (L_III) antibody production; data from 120- and 270-days old mice (named “adult” or “aged”, respectively) were compared.

Results

WT Hsp65 administration induces reduction in the mean survival time of adult and aged female H_III mice, this effect being stronger in aged individuals. Surprisingly, the native protein administration increased the survival of aged female L_III when compared to K⁴⁰⁹A and control groups. No survival differences were observed in aged male mice after Hsp65 proteins inoculation. We observed increase in IgG1 anti-Hsp65 in WT and K⁴⁰⁹A aged H_III female mice groups and no marked changes in the anti-DNA (adult and aged H_III) and anti-Hsp65 IgG1 or IgG2a isotypes production in adult H_III female and aged male mice. L_III male mice presented increased anti-DNA and anti-Hsp65 IgG2a isotype production after WT or K⁴⁰⁹A injection, and L_III female groups showed no alterations.

Conclusions

The results revealed that the WT Hsp65 interferes with survival of aged H_III female mice without involvement of a remarkable IgG1 and IgG2a anti-DNA and anti-Hsp65 antibodies production. The deleterious effects of Hsp65 on survival time in aged H_III female mice could be linked to a gender-effect and are in agreement with those previously reported in lupus-prone mice.

【 授权许可】

   
2014 Baldon et al.; licensee BioMed Central Ltd.

【 图 表 】

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