Phenotypic and functional alterations of monocyte subsets with aging

Immunity & Ageing

Research

Guoli Li¹  Yaxian Kong¹  Zengtao Wang¹  Yang Fan¹  Yu Cao¹  Chuan Song¹  Hui Zeng²  Chen Chen³  Junyan Han³  Fangyuan Li³  Yu Hao³  Dannuo Han⁴  Xing Hao⁴ 

[1] Beijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, 100015, Beijing, China;Beijing Institute of Infectious Diseases, 100015, Beijing, China;National Center for Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, 100015, Beijing, China;Beijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, 100015, Beijing, China;Beijing Institute of Infectious Diseases, 100015, Beijing, China;National Center for Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, 100015, Beijing, China;Biomedical Innovation Center, Beijing Shijitan Hospital, Capital Medical University, 100038, Beijing, China;Beijing Key Laboratory for Therapeutic Cancer Vaccines, Beijing Shijitan Hospital, Capital Medical University, 100038, Beijing, China;Biomedical Innovation Center, Beijing Shijitan Hospital, Capital Medical University, 100038, Beijing, China;Beijing Key Laboratory for Therapeutic Cancer Vaccines, Beijing Shijitan Hospital, Capital Medical University, 100038, Beijing, China;Center for Cardiac Intensive Care, Beijing Anzhen Hospital, Capital Medical University, 100029, Beijing, China;

关键词: Aging;    Monocytes;    Immunosenescence;    Activation;   

DOI  :  10.1186/s12979-022-00321-9

 received in 2022-04-07, accepted in 2022-12-02,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundIt has been widely accepted that monocytes are one of the central mediators contributing to inflammaging. However, it remains unclear whether aged monocytes, similar to aged T cells, have characteristics of hyperactivation and increased expression of co-inhibitory molecules.MethodsPeripheral blood mononuclear cells (PBMCs) were isolated from young (21–40 years old), middle-aged (41–60 years old), and older human subjects (> 60 years old). Flow cytometry was used to monitor changes in the expression of surface molecules of monocyte subsets and cytokine-producing capacity.ResultsWe observed increased tumor necrosis factor-α: TNF-α and decreased interleukin-6 (IL-6) production in monocytes from older adults compared with young and middle-aged adults. Older adults had a greater percentage of intermediate and non-classical monocyte subsets, along with increased levels of the immune activation markers human leukocyte antigen-DR (HLA-DR), and adhesion molecules cluster of differentiation molecule 11b (CD11b) and L-selectin (CD62L). Furthermore, we observed increased C–C motif chemokine receptor 2 (CCR2) expression on classical monocytes and decreased C-X3-C motif chemokine receptor 1 (CX3CR1) expression on non-classical monocytes in older adult subjects. The expression of co-inhibitory receptors was reduced on monocyte subsets in older adults.ConclusionsCirculating monocytes in older adults exhibit increased expression of activation, adhesion, and migration markers, but decreased expression of co-inhibitory molecules.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

Fig. 1

Fig. 5

Fig. 1

Fig. 1

Fig. 3

Fig. 7

Fig. 3

Fig. 2