PinX1 regulation of telomerase activity and apoptosis in nasopharyngeal carcinoma cells

Journal of Experimental & Clinical Cancer Research

Hai-Li Wang²  Ping-Neng Zhong²  Jun-Qi Wang²  Chao-Sheng Yu²  Yu-Hong Qian²  Zhong Wen¹  Cong-Xiang Shen²  Xiao-Fen Lai² 

[1] Department of Otolaryngology Head and Neck Surgery, Zhujiang Hospital, Southern Medical University, 253# GongYe Road, Guangzhou 510282, China;Department of Otolaryngology Head and Neck Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China

关键词: Human nasopharyngeal carcinoma;    Cell cycle;    Apoptosis;    Migration;    Proliferation;    Telomerase inhibitor;    PinX1;   

Others  :  826840
DOI  :  10.1186/1756-9966-31-12

 received in 2011-11-09, accepted in 2012-02-08,  发布年份 2012

【 摘 要 】

Background

Human interacting protein X1 (PinX1) has been identified as a critical telomerase inhibitor and proposed to be a putative tumor suppressor gene. Loss of PinX1 has been found in a large variety of malignancies, however, its function in inhibiting telomerase activity of tumor cells is not well documented. Here we show that PinX1 is essential for down-regulation telomerase activity of nasopharyngeal carcinoma.

Methods

Expression vectors of human PinX1 (pEGFP-C3-PinX1) and its small interfering RNA (PinX1-FAM-siRNA) were constructed and transfected into NPC. Their effects on mRNA of telomerase catalytic subunit (hTERT), telomerase activity, cell proliferation, cell migration, wound healing, cell cycles and apoptosis were examined using semi-quantitative RT-PCR, stretch PCR, MTT assay, Transwell, scratch assay and flow cytometry, respectively.

Results

Transfection of pEGFP-C3-PinX1 and PinX1-FAM-siRNA increased and reduced PinX1 mRNA by 1.6-fold and 70%, respectively. Over-expression of PinX1 decreased hTERT mRNA by 21%, reduced telomerase activity, inhibited cell growth, migration and wound healing ability, arrested cells in G0/G1 phase, and increased apoptotic index. In contrast, down-regulation of PinX1 did not alter the above characteristics.

Conclusions

PinX1 may play important roles in NPC proliferation, migration and apoptosis and has application potential in tumor-targeted gene therapy.

【 授权许可】

   
2012 Lai et al; licensee BioMed Central Ltd.

【 图 表 】

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