Changes in granulosa cells gene expression associated with growth, plateau and atretic phases in medium bovine follicles

Journal of Ovarian Research

Marc-André Sirard¹  Gabriel Douville¹ 

[1] Centre de Recherche en Biologie de la Reproduction, Faculté des Sciences de l’Agriculture et de l’Alimentation, Département des Sciences Animales, Pavillon INAF, Université Laval, Québec G1V 0A6, QC, Canada

关键词: Cow;    Growth status;    Transcriptome;    Granulosa cells;   

Others  :  801392
DOI  :  10.1186/1757-2215-7-50

 received in 2014-02-17, accepted in 2014-04-11,  发布年份 2014

【 摘 要 】

Background

The objective of this study was to build the transcriptomic profile of granulosa cells originating from follicles 6 to 9 mm in diameter in dairy cattle using microarrays.

Methods

Granulosa cells originating from three different phases of antral follicle growth were compared: growing (G), plateau (P) and atresia (A), as categorized by flow cytometry profiles of DNA. The growing and atretic conditions were each hybridized against the plateau condition as a reference in order to understand the specific biological mechanisms modulated in this class of follicles.

Results

2,942 genes were differentially expressed (P < 0.05) in P vs. G and 1,974 in A vs. P. A clear segregation of the 3 phases was confirmed by between group analysis (BGA). The first characteristic of the plateau phase is the activation of the upstream regulators TP53 and PTEN which participate in the reduction of cell growth through MYC, FOS and E2F1-2-3. We also observed the down-regulation of steroidogenesis genes: CYP11A1 and CYP19A1, in the granulosa cells of the plateau phase relative to the growth phase. On the other hand, the A vs. P contrast showed up-regulation of multiple transcripts associated to apoptosis: CCT2, DAB2, DSG2 and TGM2.

Conclusions

This study offers multiple candidate genes to be further studied in order to elucidate their role in the modulation of follicular development and, ultimately, of oocyte quality.

【 授权许可】

   
2014 Douville and Sirard; licensee BioMed Central Ltd.

【 图 表 】

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