An overview of gene expression dynamics during early ovarian folliculogenesis: specificity of follicular compartments and bi-directional dialog

BMC Genomics

Research Article

Nathalie Marsaud¹  Cedric Cabau²  Beatrice Mandon-Pepin³  Agnes Bonnet⁴  Philippe Mulsant⁴  Sylvain Foissac⁴  Florent Woloszyn⁴  Julien Sarry⁴  Olivier Bouchez⁵ 

[1] GeT-PlaGe, Genotoul, INRA Auzeville, F31326, Castanet-tolosan, France;Université de Toulouse; INSA, UPS, INP; LISBP, 135 Avenue de Rangueil, F-31077, Toulouse, France;INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, F-31400, Toulouse, France;CNRS, UMR5504, F-31400, Toulouse, France;INRA, SIGENAE, UR83 Recherches Avicoles, F-37380, Nouzilly, France;INRA, UMR1198 Biologie du Développement et de la Reproduction, F-78350, Jouy-en-Josas, France;INRA, UMR444 Génétique Cellulaire, Auzeville, BP52627, F-31326, Castanet-Tolosan, France;ENVT, UMR444, Laboratoire de Génétique Cellulaire, F-31326, Castanet-Tolosan, France;INRA, UMR444 Génétique Cellulaire, Auzeville, BP52627, F-31326, Castanet-Tolosan, France;ENVT, UMR444, Laboratoire de Génétique Cellulaire, F-31326, Castanet-Tolosan, France;GeT-PlaGe, Genotoul, INRA Auzeville, F31326, Castanet-tolosan, France;

关键词: RNA-seq;    Early folliculogenesis;    Molecular dialog;    Transcriptome;    Sheep;    Oocyte;    Granulosa cells;    Microdissection;   

DOI  :  10.1186/1471-2164-14-904

 received in 2013-06-06, accepted in 2013-12-10,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundSuccessful early folliculogenesis is crucial for female reproductive function. It requires appropriate gene specific expression of the different types of ovarian cells at different developmental stages. To date, most gene expression studies on the ovary were conducted in rodents and did not distinguish the type of cell. In mono-ovulating species, few studies have addressed gene expression profiles and mainly concerned human oocytes.ResultsWe used a laser capture microdissection method combined with RNA-seq technology to explore the transcriptome in oocytes and granulosa cells (GCs) during development of the sheep ovarian follicle. We first documented the expression profile of 15 349 genes, then focused on the 5 129 genes showing differential expression between oocytes and GCs. Enriched functional categories such as oocyte meiotic arrest and GC steroid synthesis reflect two distinct cell fates. We identified the implication of GC signal transduction pathways such as SHH, WNT and RHO GTPase. In addition, signaling pathways (VEGF, NOTCH, IGF1, etc.) and GC transzonal projections suggest the existence of complex cell-cell interactions. Finally, we highlighted several transcription regulators and specifically expressed genes that likely play an important role in early folliculogenesis.ConclusionsTo our knowledge, this is the first comprehensive exploration of transcriptomes derived from in vivo oocytes and GCs at key stages in early follicular development in sheep. Collectively, our data advance our understanding of early folliculogenesis in mono-ovulating species and will be a valuable resource for unraveling human ovarian dysfunction such as premature ovarian failure (POF).

【 授权许可】

CC BY   
© Bonnet et al.; licensee BioMed Central Ltd. 2013