Canine testicular tumors: two types of seminomas can be differentiated by immunohistochemistry

BMC Veterinary Research

Željko Grabarević¹  Ivan-Conrado Šoštarić-Zuckermann¹  Ana Beck¹  Andrea Gudan Kurilj¹  Krešimir Severin²  Branka Artuković¹  Marko Hohšteter¹ 

[1] Department of Veterinary Pathology, Veterinary Faculty, University of Zagreb, Heinzelova 55, Zagreb 10 000, Croatia;Department of Forensic and Judicial Veterinary Medicine, Veterinary Faculty, University of Zagreb, Heinzelova 55, Zagreb 10 000, Croatia

关键词: IGCNU;    Incidence;    Immunohistochemistry;    Seminoma;    Tumors;    Testis;    Dog;   

Others  :  1119101
DOI  :  10.1186/s12917-014-0169-8

 received in 2013-12-17, accepted in 2014-07-21,  发布年份 2014

【 摘 要 】

Background

Testicular tumors are the most common genital neoplasms in male dogs, with Leydig cell tumors (LCT), seminomas (SEM), and Sertoli cell tumors (SCT) the most common forms. Human SEM are classified as classical (CSEM) or spermatocytic (SSEM). Intratubular germ cell neoplasia of undifferentiated origin (IGCNU) is another form of human testicular tumor. The aim of this study was to verify that CSEM/SSEM classification is valid in dogs and confirm the existence of canine IGCNU.

Results

Testicular tumors were found in 46% of dogs at necropsy and accounted for 7% of tumors biopsied. The median age of dogs with tumors at necropsy was 10.16 years; median age at positive biopsy was 10.24 years. The most common tumors, in decreasing order, were LCT, mixed tumors, SEM and SCT at necropsy, and SEM, SCT, mixed tumors, LCT, peripheral nerve sheath tumor, and teratoma in the biopsy group. IGCNU was found in 3% of testicles at necropsy and in 3% of biopsy samples. Two dogs had testicular tumor metastasis. Expression of c-KIT was most common in SEM and seminomatous components of mixed tumors. PLAP was mostly expressed in IGCNU, SEM, teratoma, and some mixed tumors. Cytokeratin was mainly expressed in SCT. CD30 expression was low in both groups.

Conclusions

The high tumor incidence at necropsy can be attributed to older age. Tumor incidence in biopsy samples, dog age, and histological classification were consistent with previous studies. The higher incidence of SEM and SCT in the biopsy group probably resulted from the obvious clinical expression of these tumor types. The low incidence of metastasis confirmed the predominance of benign tumors. Low CD30 expression confirmed the low incidence of testicular embryonal carcinoma. Cytokeratin helps differentiate stromal tumors, especially SCT, from germ cell tumors. Histology and c-KIT and PLAP expression indicate that IGCNU exists in dogs. Expression of c-KIT and PLAP confirmed that CSEM and SSEM classification is valid in dogs.

【 授权许可】

   
2014 Hohsteter et al.; licensee BioMed Central Ltd

【 图 表 】

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