Genome sequencing, annotation and comparative genomic analysis of Shigella dysenteriae strain SD1D

Gut Pathogens

Shanmugam Mayilraj²  Javed N Agrewala¹  Nida Mubin¹  Sheenam Verma¹  Amit Arora²  Sathyaseelan Sathyabama¹  Gurwinder Kaur² 

[1] Immunology Laboratory, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India;Microbial Type Culture Collection and Gene bank, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India

关键词: RAST;    EzTaxon;    G + C content;    Shigellosis;    Shigella dysenteriae;   

Others  :  1133847
DOI  :  10.1186/1757-4749-6-28

 received in 2014-05-13, accepted in 2014-07-02,  发布年份 2014

【 摘 要 】

Background

Shigellosis is an acute form of gastroenteritis caused by the bacteria belonging to the genus Shigella. It is the most common cause of morbidity and mortality in children. Shigella belongs to the family Enterobactericeae, which is a Gram-negative and rod shaped bacterium. In the present study, we report the draft genome of Shigella dysenteriae strain SD1D, which was isolated from the stool sample of a healthy individual.

Results

Based on 16S rRNA gene sequence and phylogenetic analysis, the strain SD1D was identified as Shigella dysenteriae. The draft genome of SD1D consisted of 45, 93, 159 bp with a G + C content of 50.7%, 4, 960 predicted CDSs, 75 tRNAs and 2 rRNAs. The final assembly contained 146 contigs of total length 45, 93, 159 bp with N₅₀ contig length of 77, 053 bp; the largest contig assembled measured 3, 85, 550 bp.

Conclusions

We have for the first time performed the whole genome sequencing of Shigella dysenteriae strain SD1D. The comparative genomic analysis revealed several genes responsible for the pathogenesis, virulence, defense, resistance to antibiotics and toxic compounds, multidrug resistance efflux pumps and other genomic features of the bacterium.

【 授权许可】

   
2014 Kaur et al.; licensee BioMed Central Ltd.

【 图 表 】

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