Der f 16: a novel gelsolin‐related molecule identified as an allergen from the house dust mite, Dermatophagoides farinae 1

FEBS Letters

Kawamoto, Seiji²  Tsuboi, Shinji¹  Ono, Kazuhisa²  Shigeta, Seiko²  Katsutani, Takashi³  Suzuki, Takayuki²  Aki, Tsunehiro² 

[1] Tsuboi Clinic, Otake, Japan;Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan;Katsutani Clinic, Hatsukaichi, Japan

关键词: Allergen;    Asthma;    Gelsolin;    House dust mite;    IgE;    Dfb;    Dermatophagoides farinae body extract;    GST;    glutathione S-transferase;    IPTG;    isopropyl-1-thio-β-D-galactoside;    PIP2;    polyphosphoinositide 4;    5-biphosphate;    RAST;    radioallergosorbent test;   

DOI  :  10.1016/S0014-5793(02)02540-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Allergen from the house dust mite (Dermatophagoides sp.) is a major trigger factor of allergic disorders, and its characterization is crucial for the development of specific diagnosis or immunotherapy. Here we report the identification of a novel dust mite (Dermatophagoides farinae) antigen whose primary structure belongs to the gelsolin family, a group of actin cytoskeleton-regulatory proteins. Isolated mite cDNA, termed Der f 16, encodes 480 amino acids comprising a four-repeated gelsolin-like segmental structure, which is not seen in conventional gelsolin family members. Enzyme immunoassay indicated that recombinant Der f 16 protein, prepared using an Escherichia coli expression system, bound IgE from mite-allergic patients at 47% (8/17) frequency. This is the first evidence that the gelsolin family represents a new class of allergen recognizable by atopic patient IgE.

【 授权许可】

Unknown