Global differences in specific histone H3 methylation are associated with overweight and type 2 diabetes

Clinical Epigenetics

Peter Strålfors¹  Alexander V Vener¹  Risul Amin¹  Kim Lundqvist¹  Simon Sjödin¹  Åsa Jufvas¹ 

[1] Department of Clinical and Experimental Medicine, Linköping University, Linköping SE58185, Sweden

关键词: Type 2 diabetes;    Primary mature adipocytes;    Overweight;    Obesity;    Human;    Histone methylation;    Epigenetic;   

Others  :  790894
DOI  :  10.1186/1868-7083-5-15

 received in 2013-05-01, accepted in 2013-07-12,  发布年份 2013

【 摘 要 】

Background

Epidemiological evidence indicates yet unknown epigenetic mechanisms underlying a propensity for overweight and type 2 diabetes. We analyzed the extent of methylation at lysine 4 and lysine 9 of histone H3 in primary human adipocytes from 43 subjects using modification-specific antibodies.

Results

The level of lysine 9 dimethylation was stable, while adipocytes from type 2 diabetic and non-diabetic overweight subjects exhibited about 40% lower levels of lysine 4 dimethylation compared with cells from normal-weight subjects. In contrast, trimethylation at lysine 4 was 40% higher in adipocytes from overweight diabetic subjects compared with normal-weight and overweight non-diabetic subjects. There was no association between level of modification and age of subjects.

Conclusions

The findings define genome-wide molecular modifications of histones in adipocytes that are directly associated with overweight and diabetes, and thus suggest a molecular basis for existing epidemiological evidence of epigenetic inheritance.

【 授权许可】

   
2013 Jufvas et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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