NCAM (CD56) Expression in keratin-producing odontogenic cysts: aberrant expression in KCOT

Head & Face Medicine

Francisco Vera-Sempere²  Leopoldo Forner-Navarro¹  Beatriz Vera-Sirera¹ 

[1] Departaments of Stomatology, University of Valencia, Valencia, Spain;Service of Pathology, Hospital Universitario y Politécnico La Fe, Avda Campanar 21, Valencia 46009, Spain

关键词: Immunohistochemistry;    Orthokeratinized odontogenic cyst;    Keratocystic odontogenic tumor;    Keratocysts;    NCAM;   

Others  :  1133794
DOI  :  10.1186/s13005-015-0060-2

 received in 2014-09-10, accepted in 2015-01-20,  发布年份 2015

【 摘 要 】

Objective

To investigate immunohistochemically the expression of neural cell adhesion molecule (NCAM), which has been identified as a signaling receptor with frequent reactivity in ameloblastomas (AB), in a series of keratin-producing odontogenic cysts (KPOCs).

Material and methods

Immunohistochemical expression of NCAM, using a monoclonal antibody, was determined in a series of 58 KPOCs comprising 12 orthokeratinized odontogenic cysts (OOCs) and 46 keratocystic odontogenic tumors (KCOTs), corresponding to 40 non-syndromic KCOT (NS-KCOTs) and 6 syndromic KCOT (S-KCOTs), associated with nevic basocellular syndrome (NBCS).

Results

NCAM expression was negative in all OOCs, but 36.45% of KCOTs exhibited focal and heterogeneous expression at the basal cell level, as well as in basal budding areas and the basal cells of daughter cysts. The latter two locations were especially applicable to S-KCOTs, with focal NCAM reactivity occurring in 66.66% of cases.

Conclusions

Aberrant NCAM expression, in KCOTs but especially in S-KCOTs, together with its immunomorphological location, suggests that this adhesion molecule and signaling receptor plays a role in the pathogenesis of KCOTs, with a probable impact on lesional recurrence.

【 授权许可】

   
2015 Vera-Sirera et al. licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

【 参考文献 】

• [1]Shear M: The aggressive nature of the odontogenic keratocyst: it is a benign cystic neoplasm? part 1. clinical and early experimental evidence of aggressive behaviour. Oral Oncol 2002, 38:219-26.
• [2]Barnes L, Eveson JW, Reichart P, Sidransky D: World health organization classification of tumours. IARC, Lyon; 2005.
• [3]Johnson NR, Batstone MD, Savage NW: Management and recurrence of keratocystic odontogenic tumor: a systematic review. Oral Surg Oral Med Oral Pathol Oral Radiol 2013, 116:e271-6.
• [4]Barghava D, Deshpande A, Pogrel MA: Keratocystic odontogenic tumor (KCOT) - a cyst to a tumour. Oral Maxillofac Surg 2012, 16:163-70.
• [5]Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH: Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM / CD56). J Immunol 1991, 146:4421-6.
• [6]Kishimoto T, Kikutani H, von der Borne AEGK, Goyert SM, Mason DY, Miyasaka M, et al.: Leukocyte typing VI: white cell differentiation antigens. Garland, New York; 1997.
• [7]Gattenlöhner S, Stühmer T, Leich E, Reinhard M, Etschmann B, Völker HU, et al.: Specific detection of CD56 (NCAM) isoforms for the identification of aggressive malignant neoplasms with progressive development. Am J Pathol 2009, 174:1160-71.
• [8]Er N, Dagdevirem A, Tasman F, Zeibek D: Neural cell adhesion molecule and neurothelin expression in human ameloblastoma. J Oral Maxillofac Surg 2001, 59:900-3.
• [9]Kawai S, Ito E, Yamaguchi A, Eishi E, Okada N: Immunohistochemical characteristics of odontogenic carcinomas: their use in diagnosing and elucidating histognesis. Oral Med Pathol 2009, 13:55-63.
• [10]Kusafuka K, Hirobe K, Wato M, Tanaka A, Nakajima T: CD56 expression is associated with neuroectodermal differentiation in ameloblastomas: an immunohistochemical evaluation in comparison with odontogenic cystic lesions. Med Mol Morphol 2011, 44:79-85.
• [11]Cairns L, Naidu A, Robinson CM, Sloan P, Wright JM, Hunter KD: CD56 (NCAM) expression in ameloblastomas and other odontogenic lesions. Histopathology 2010, 57:544-8.
• [12]Nayak MT, Singh A, Singhvi A, Sharma R: Odontogenic keratocyst: what is the name? J Nat Sci Biol Med 2013, 4:282-5.
• [13]Woolgar JA, Rippin JW, Browne RM: A comparative study of the clinical and histological features of recurrent and non-recurrent odontogenic keratocysts. J Oral Pathol 1987, 16:124-8.
• [14]Kimonis VE, Goldstein AM, Pastakia B: Clinical manifestations in 105 persons with nevoid basal cell carcinoma syndrome. Am J Med Genet 1997, 69:299-308.
• [15]Obara N, Suzuki Y, Nagai Y, Nishiyama H, Mizoguchi I, Takeda M: Expression of neural cell-adhesion molecule mRNA during mouse molar tooth development. Arch Oral Biol 2002, 47:805-13.
• [16]Wright JM: The odontogenic keratocyst: orthokeratinized variant. Oral Surg 1981, 51:609-18.
• [17]Dong Q, Pan S, Sun LS, Li TJ: Orthokeratinized odontogenic cyst. a clinicopathological study of 61 cases. Arch Pathol Lab Med 2010, 134:271-5.
• [18]Regezi JA: Odontogenic cysts, odontogenic tumors, fibroosseous, and giant cell lesions of the jaws. Mod Pathol 2002, 15:331-41.
• [19]Kuroyanagi N, Sakuma H, Miyabe S, Machida J, Kaetsu A, Yokoi M, et al.: Prognostic factors for keratocystic odontogenic tumor (odontogenic keratocyst): analysis of clinico-pathologic and immunohistochemical findings in cysts treated by enucleation. J Oral Pathol Med 2009, 38:386-92.
• [20]Mendes RA, Carvalho JFC, van der Waal I: Characterization and management of the keratocystic odontogenic tumor in relation to its histopathological and biological features. Oral Oncol 2010, 46:219-25.
• [21]Myoung H, Hong SP, Hong SD, Lee JI, Lim CY, Choung PH, et al.: Odontogenic keratocyst: review of 256 cases for recurrence and clinicopathological parameters. Oral Surg Oral Med Oral Pathol 2001, 91:328-33.
• [22]Woolgar JA, Rippin JW, Browne RM: A comparative histological study of odontogenic keratocysts in basal cell naevus syndrome and control patients. J Oral Pathol 1987, 16:75-80.
• [23]MacDonal-Jankowsk DS: Keratocystic odontogenic tumor: systematic review. Dentomaxillofac Radiol 2011, 40:1-23.
• [24]Lo Muzio L, Staibano S, Pannone G, Bucci P, Nocini PF, Bucci E, et al.: Expression of cell cycle and apoptosis-related proteins in sporadic odontogenic keratocysts and odontogenic keratocysts associated with the nevoid basal cell carcinoma syndrome. J Dent Res 1999, 78:1345-53.
• [25]Zecha JAEM, Mendes RA, Lindeboom VB, Van der Waal I: Recurrence rate of keratocystic odontogenic tumor after conservative surgical treatment without adjunctive therapies – A 35-year single institution experience. Oral Oncol 2010, 46:740-2.
• [26]Oliveira MG, Lauxen IS, Filho MSA: P53 protein reactivity in odontogenic lesions: an immunohistochemical study. R Fac Odonto Porto Alegre 2005, 46:31-5.
• [27]Vera-Sirera B, Forner-Navarro L, Vera-Sempere F: Differential expression of cyclin D1 in keratin-producing odontogenic cysts. Med Oral Patol Oral Cir Bucal 2015, 20:e59-65.
• [28]Heikinheimo K, Jee PR, Morgan N, Nagy S, Knuutila S, Leivo I: Genetic changes in sporadic keratocystic odontogenic tumors (odontogenic keratocysts). J Dent Res 2007, 86:544-9.
• [29]Eversole LR, Sabes WR, Rovin S: Aggressive growth and neoplastic potential of odontogenic cysts: with special reference to central epidermoid and mucoepidermoid carcinomas. Cancer 1975, 35:270-82.
• [30]Ide F, Ito Y, Muramatsu T, Saito I, Abiko Y, et al.: Histogenetic relations between keratoameloblastoma and solid variant of odontogenic keratocyst. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2012, 114:812-3.
• [31]Whitt JC, Dunlap CL, Sheets JL, Thompson ML: Keratoameloblastoma: a tumor sui generis or a chimera? Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007, 104:368-76.
• [32]Geng N, Lv D, Chen QM, Zhu ZY, Wu RQ, He ZX: Solid variant of keratocystic odontogenic tumor with ameloblastomatous transformation: a case report and review of the literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2012, 114:223-9.
• [33]Ketabi MA, Dehghani N, Sadeghi HM, Shams MG, Mohajerani H, Azarsina M, et al.: Keratoameloblastoma, a very rare variant of ameloblastoma. J Craniofac Surg 2013, 24:2182-6.
• [34]Geng N, Chem Q-M: Differentiating solid variants of keratocystic odontogenic tumors and keratoameloblastomas. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2013, 114:813-4.