Dietary saturated and monounsaturated fats protect against acute acetaminophen hepatotoxicity by altering fatty acid composition of liver microsomal membrane in rats

Lipids in Health and Disease

Hye Jin Hwang⁵  Eugene Shim¹  Haeyon Chung⁴  Soo Yeon Kim⁴  Jung Hwa Park²  Yun-Hee Chang³  Jinah Hwang³ 

[1] National Hypertension Center, Yonsei University Health System, Seoul 120-752, Republic of Korea;Research Institute of Food & Nutritional Sciences, Yonsei University, Seoul, 120-749, Republic of Korea;Department of Foods and Nutrition, College of Natural Sciences, Myongji University, YongIn 449-728, Republic of Korea;Department of Food and Nutrition, Division of Natural Sciences, Seoul, 100-751, Republic of Korea;Department of Food and Nutrition, College of Human Ecology, Dongeui University, Busan 614-714, Republic of Korea

关键词: Cytochrome P-450 2E1;    Hepatotoxicity;    MUFA;    SFA;    Phospholipid fatty acid composition;   

Others  :  1212476
DOI  :  10.1186/1476-511X-10-184

 received in 2011-06-24, accepted in 2011-10-20,  发布年份 2011

【 摘 要 】

Background

Dietary polyunsaturated fats increase liver injury in response to ethanol feeding. We evaluated the effect of dietary corn oil (CO), olive oil (OO), and beef tallow (BT) on fatty acid composition of liver microsomal membrane and acute acetaminophen hepatotoxicity.

Methods

Male Sprague-Dawley rats were fed 15% (wt/wt) CO, OO or BT for 6 weeks. After treatment with acetaminophen (600 mg/kg), samples of plasma and liver were taken for analyses of the fatty acid composition and toxicity.

Results

Treatment with acetaminophen significantly elevated levels of plasma GOT and GPT as well as hepatic TBARS but reduced hepatic GSH levels in CO compared to OO and BT groups. Acetaminophen significantly induced protein expression of cytochrome P450 2E1 in the CO group. In comparison with the CO diet, lower levels of linoleic acid, higher levels of oleic acids and therefore much lower ratios of linoleic to oleic acid were detected in rats fed OO and BT diets.

Conclusions

Dietary OO and BT produces similar liver microsomal fatty acid composition and may account for less severe liver injury after acetaminophen treatment compared to animals fed diets with CO rich in linoleic acid. These findings imply that types of dietary fat may be important in the nutritional management of drug-induced hepatotoxicity.

【 授权许可】

   
2011 Hwang et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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