Analysis of angiogenic markers in oral squamous cell carcinoma-gene and protein expression

Head & Face Medicine

Johannes Kleinheinz¹  Yahya Acil³  Christoph Sproll²  Nikolai Purcz³  Sonja Sielker¹  Susanne Jung¹ 

[1] Vascular Biology of Oral Structures (VABOS) Research Unit, Department of Cranio-Maxillofacial Surgery, University Hospital Muenster, Waldeyerstraße 30, Muenster, D-48149, Germany;Department of Cranio-Maxillofacial Surgery, University Hospital Duesseldorf, Duesseldorf, Germany;Department of Cranio-Maxillofacial Surgery, University Hospital Kiel, Kiel, Germany

关键词: Angiogenesis;    EFNB2;    VEGF;    Angiopoietins;    Microarray analysis;    Oral squamous cell carcinoma;   

Others  :  1219630
DOI  :  10.1186/s13005-015-0076-7

 received in 2014-09-11, accepted in 2015-05-20,  发布年份 2015

【 摘 要 】

Purpose

Therapeutic strategies attacking oral squamous cell carcinoma have not essentially succeeded to improve long-term prognosis and overall survival over the last decades. Therefore, in this study, we aimed to illuminate the molecular regulation of angiogenesis in this tumour entity in order to demask novel markers of prognosis or therapeutic approach.

Materials and methods

A panel of significant transcriptional alterations in angiogenic genes of 83 cancer samples was established by comparison to 30 samples of healthy oral mucosa with microarray technique. Immunohistochemistry (IHC) was performed to trace the signalling cascade from gene to protein level.

Results

A distinctive expression profile of VEGFA, EFNB2, PECAM1/CD31, ANGPT1 and ANGPT2 was revealed: VEGFA, EFNB2, and ANGPT2 were found overexpressed in 84 % to 95 % of tumour samples. In contrast, the expression of CD31 and ANGPT1 was downregulated in 80 % to 95 % of tumour samples. IHC confirmed results of the microarray analysis.

Tumours with lymphatic spread showed higher gene expression rates of VEGFA, EFNB2 and ANGPT2 in moderately differentiated tumours and of VEGFA and EFNB2 in small tumours, respectively. The ANGPT1/ ANGPT2 transcription ratio was found decreased in larger tumours and especially in tumours without lymphatic spread.

Conclusions

A characteristic expression profile of angiogenic markers was established. The specific overexpression of EFNB2 in small tumours with lymphatic spread and the typical decrease of the ANGPT1/ ANGPT2 ratio in larger tumours give weight to EFNB2 and angiopoietins as prognostic factors and potential therapeutic targets.

【 授权许可】

   
2015 Jung et al.

【 图 表 】

Fig. 1.

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