Use of in vivo induced antigen technology to identify genes from Aeromonas salmonicida subsp. salmonicida that are specifically expressed during infection of the rainbow trout Oncorhynchus mykiss

BMC Veterinary Research

Mansour El-Matbouli²  Gokhlesh Kumar²  Hatem Soliman¹  Simon Menanteau-Ledouble² 

[1] Fish Medicine and Managements, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut, 71515, Egypt;Clinical Division of Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinarplatz 1, Vienna 1210, Austria

关键词: Transcription levels;    Virulence;    TonB;    RpoD;    UDP deacetylase;    Antigenic profile;    IVIAT;   

Others  :  1091747
DOI  :  10.1186/s12917-014-0298-0

 received in 2014-06-30, accepted in 2014-12-08,  发布年份 2014

【 摘 要 】

Background

Aeromonas salmonicida is a major fish pathogen associated with mass mortalities in salmonid fish. In the present study, we applied In Vivo Induced Antigen Technology (IVIAT), a technique that relies on antibodies adsorbed against in vitro cultures of the pathogen, to a clinical isolate of A. salmonicida subsp. salmonicida.

Results

The results from IVIAT allowed identification of four proteins that were upregulated in the fish samples: A UDP-3-O-acyl-N-acetylglucosamine deacetylase, an RNA polymerase sigma factor D as well as TonB and a hypothetical protein. Subsequent investigations were performed using real-time PCR and cDNA synthesised from infected spleen, liver and anterior kidneys. These confirmed that the transcription level of each of these genes was significantly upregulated during the infection process compared to bacteria in vitro.

Conclusions

The present studied identified four genes that were upregulated during the infectious process and are likely to play a role in the virulence of A. salmonicida. Because these are antigenic they might constitute potential targets for the development of new vaccine as well as therapeutic agents.

【 授权许可】

   
2014 Menanteau-Ledouble et al.; licensee BioMed Central Ltd.

【 图 表 】

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