Quorum Sensing Activity of Aeromonas Caviae Strain YL12, A Bacterium Isolated from Compost

Sensors

Yan-Lue Lim¹  Robson Ee¹  Wai-Fong Yin¹ 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;

关键词: Cell-to-cell communication;    C4-HSL (N-butyryl-L-homoserine lactone);    C6-HSL (N-hexanoyl-L-homoserine lactone);    N-acyl homoserine lactone (AHL);    MALDI-TOF;    triple quodruopole liquid chromatography mass spectrometry (LCMS/MS);    Pacific Biosciences;    RpoD;    thin liquid chromatography (TLC);   

DOI  :  10.3390/s140407026

来源: mdpi

【 摘 要 】

Quorum sensing is a well-studied cell-to-cell communication method that involves a cell-density dependent regulation of genes expression mediated by signalling molecules. In this study, a bacterium isolated from a plant material compost pile was found to possess quorum sensing activity based on bioassay screening. Isolate YL12 was identified using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and molecular typing using rpoD gene which identified the isolate as Aeromonas caviae. High resolution tandem mass spectrometry was subsequently employed to identify the N-acyl homoserine lactone profile of Aeromonas caviae YL12 and confirmed that this isolate produced two short chain N-acyl homoserine lactones, namely C4-HSL and C6, and the production was observed to be cell density-dependent. Using the thin layer chromatography (TLC) bioassay, both AHLs were found to activate C. violaceum CV026, whereas only C6-HSL was revealed to induce bioluminescence expression of E. coli [pSB401]. The data presented in this study will be the leading steps in understanding the role of quorum sensing in Aeromonas caviae strain YL12.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.