GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions

Saudi Journal of Biological Sciences

Thu Thi Mai Lo¹  Yen Thi Hai Nguyen²  Phutthakone Vaciaxa³  Tan Quang Tu³  Phat Tien Do³  Mau Hoang Chu³  Quan Huu Nguyen³  Nhung Hong Nguyen⁴  Lan Thi Ngoc Nguyen⁵  Nhan Thi Thanh Pham⁵ 

[1] Khangkhay Teacher Training College, Xiangkhoang, Laos;VAST – Graduate University of Sciences and Technology, Hanoi, Viet Nam;TNU – University of Education, Thai Nguyen, Viet Nam;Tay Bac University, Son La, Viet Nam;VAST – Institute of Biotechnology, Hanoi, Viet Nam;

关键词: GmDREB6;    NtP5CS;    NtCLC;    Salt tolerance;    Transcription levels;   

DOI  :  

来源: DOAJ

【 摘 要 】

Soil is contaminated with salinity, which inhibits plant growth and development and reduces crop yields. The DREB (dehydration responsive element binding protein) gene responds to salt stresses through enhanced transcriptional expression and activation of genes involved in plant salinity resistance. In this study, we present the results of the analysis of the expression of the GmDREB6 transgene, a gene that encodes the soybean DREB6 transcription factor, regulating the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions. The transcription of GmDREB6, NtP5CS, and NtCLC in transgenic tobacco lines was confirmed by qRT-PCR. Under salt stress conditions, the GmDREB6 gene transcription levels in the transgenic tobacco lines L1 and L9 had increased from 2.40- to 3.22- fold compared with the condition without salinity treatment. Two transgenic lines, L1 and L9, had transcription levels of the P5CS gene that had increased from 1.24- to 3.60- fold compared with WT plants. For the NtCLC gene, under salt stress conditions, the transgenic lines had transcription levels that had increased by 3.65–4.54 (fold) compared with WT plants (P < 0.05). The L1-transgenic tobacco line showed simultaneous expression of both the GmDREB6 transgene and two intrinsic genes, the NtP5CS and NtCLC genes. This study demonstrated that expression of the GmDREB6 gene from soybean increases the transcription levels of the NtP5CS and NtCLC genes in transgenic tobacco plants under salt stress conditions. The analysis results have suggested that the GmDREB6 gene is a potential candidate for improving the salt tolerance of plants, opening up research and development opportunities for salt stress-tolerant crops to respond to climate change and the rise in sea levels.

【 授权许可】

Unknown