Virological and clinical characterizations of respiratory infections in hospitalized children

Italian Journal of Pediatrics

Öznur Küçük¹  Çigdem Kaspar²  Gülden Çelik³  Yesim Gürol³  Ayça Vitrinel¹  Gülay Çiler Erdağ¹  Defne Çöl¹  Tuba Giray¹  Suat Bicer¹ 

[1] Yeditepe University, Faculty of Medicine, Department of Child Health and Diseases, Yeditepe University Hospital, Devlet Yolu Ankara Cad. No: 102-104,, Istanbul, Ataşehir, 34752, Turkey;Yeditepe University, Faculty of Medicine, Department of Medical Statistics, Yeditepe University Hospital, Devlet Yolu Ankara Cad. No: 102-104, Istanbul, Ataşehir, 34752, Turkey;Yeditepe University, Faculty of Medicine, Department of Medical Microbiology, Yeditepe University Hospital, Devlet Yolu Ankara Cad. No: 102-104,, Istanbul, Ataşehir, 34752, Turkey

关键词: Respiratory viruses;    Viral respiratory pathogens;    Respiratory infections;    PCR;    Hospitalization;    Child;   

Others  :  825252
DOI  :  10.1186/1824-7288-39-22

 received in 2012-11-15, accepted in 2013-03-21,  发布年份 2013

【 摘 要 】

Background

The purpose of this study was to determine the incidence and seasonal distribution of viral etiological agents and to compare their clinical manifestations and disease severity, including single and co infections.

Methods

Multiplex reverse-transcription PCR was performed for the detection of viruses in nasopharyngeal aspirat. Disease severity was grouped using a categorization index as very mild/mild, and moderate/severe. Clinical and laboratory characteristics of hospitalized children with viral respiratory tract infection were analyzed.

Results

Viral pathogens were detected in 103/155 (66.5%) of patients. In order of frequency, identified pathogens were respiratory syncytial virus (32.0%), adenovirus (26.2%), parainfluenza viruses type 1–4 (19.4%), rhinovirus (18.4%), influenza A and B (12.6%), human metapneumovirus (12.6%), coronavirus (2.9%), and bocavirus (0.9%). Coinfections were present in 21 samples. Most of the children had very mild (38.8%) and mild disease (37.9%). Severity of illness was not worse with coinfections. The most common discharge diagnoses were "URTI" with or without LRTI/asthma (n=58). Most viruses exhibited strong seasonal patterns. Leukocytosis (22.2%) and neutrophilia (36.6%) were most commonly detected in patients with adenovirus and rhinovirus (p<0.05). Monocytosis was the most remarkable finding in the patients (n=48, 53.3%), especially in patients with adenovirus (p<0.05).

Conclusions

RSV and RhV were associated with higher severity of illness in hospitalized children. RSV found to account for half of LRTI hospitalizations. In AdV and FluA and B infections, fever lasted longer than in other viruses. Coinfections were detected in 21 of the patients. The presence of coinfections was not associated with increased disease severity.

【 授权许可】

   
2013 Bicer et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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