Spermine reverses lipopolysaccharide-induced memory deficit in mice

Journal of Neuroinflammation

Maribel Antonello Rubin²  Carlos Fernando Mello¹  Thiago Duarte³  Lediane Tomazi³  Rafael Porto Ineu³  Pâmella Karina Santana Frühauf³ 

[1] Department of Physiology and Pharmacology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil;Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, Camobi, Santa Maria, CEP: 97105900, RS, Brazil;Graduation Program in Pharmacology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil

关键词: Spermine;    Polyamines;    NMDA receptor object recognition;    Neuroinflammation;    Memory;    Ifenprodil;   

Others  :  1133403
DOI  :  10.1186/s12974-014-0220-5

 received in 2014-07-24, accepted in 2014-12-11,  发布年份 2015

【 摘 要 】

Background

Lipopolysaccharide (LPS) induces neuroinflammation and memory deficit. Since polyamines improve memory in various cognitive tasks, we hypothesized that spermine administration reverses LPS-induced memory deficits in an object recognition task in mice. The involvement of the polyamine binding site at the N-methyl-D-aspartate (NMDA) receptor and cytokine production in the promnesic effect of spermine were investigated.

Methods

Adult male mice were injected with LPS (250 μg/kg, intraperitoneally) and spermine (0.3 to 1 mg/kg, intraperitoneally) or ifenprodil (0.3 to 10 mg/kg, intraperitoneally), or both, and their memory function was evaluated using a novel object recognition task. In addition, cortical and hippocampal cytokines levels were measured by ELISA four hours after LPS injection.

Results

Spermine increased but ifenprodil decreased the recognition index in the novel object recognition task. Spermine, at doses that did not alter memory (0.3 mg/kg, intraperitoneally), reversed the cognitive impairment induced by LPS. Ifenprodil (0.3 mg/kg, intraperitoneally) reversed the protective effect of spermine against LPS-induced memory deficits. However, spermine failed to reverse the LPS-induced increase of cortical and hippocampal cytokine levels.

Conclusions

Spermine protects against LPS-induced memory deficits in mice by a mechanism that involves GluN2B receptors.

【 授权许可】

   
2015 Frühauf et al.; licensee BioMed Central.

【 图 表 】

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