Kaempferol pretreatment modulates systemic inflammation and oxidative stress following hemorrhagic shock in mice

Chinese Medicine

Shao-Zhou Ni²  Peng Xu²  Jun Shen²  Yan Zhao²  Xian-Long Zhou²  Li-Ping He¹  Qi-Sheng Yang² 

[1] Department of Anesthesiology, Tong Cheng Hospital, Tongcheng 437400, Hubei, China;Emergency Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, China

关键词: Systematic inflammation;    Oxidative stress;    Kaempferol;    Hemorrhagic shock;   

Others  :  1161122
DOI  :  10.1186/s13020-015-0035-z

 received in 2014-05-26, accepted in 2015-03-06,  发布年份 2015

【 摘 要 】

Background

Kaempferol has been reported as beneficial for both acute and chronic inflammatory diseases. This study aims to investigate whether kaempferol affects systemic inflammation and oxidative stress in the heart, lung, and liver after hemorrhagic shock in mice.

Methods

Male C57/BL6 mice underwent hemorrhagic shock (mean arterial pressure of 35 mmHg for 90 min) and were arbitrarily divided into Sham, hemorrhagic shock (HS), and Kae groups (n = 10 in each group). Mice in the Kae groups received a kaempferol (10-mg/kg body weight) injection 12 h prior to (Group Kae PT) or 90 min after (Group Kae T) the initiation of hemorrhagic shock. Plasma proinflammatory cytokines (TNF-α and IL-6), organ myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, and organ malondialdehyde (MDA) concentrations and heme oxygenase-1 (HO-1) expression levels were assessed by enzyme-linked immunosorbent assay (ELISA) or western blot assay.

Results

Compared with the HS group and the Kae T group, pretreatment with kaempferol significantly decreased proinflammatory cytokines TNF-α (P = 0.012 and 0.015, respectively) and IL-6 (P = 0.023 and 0.014, respectively) following hemorrhagic shock. Kae pretreatment reverted MPO, SOD, and MDA to basal levels in the heart, lung, and liver (Ps < 0.05), while the Kae T group showed no significant differences in these biomarkers compared with the HS group (Ps > 0.05). HO-1 expression was significantly increased in the Kae PT group compared with the other groups (P = 0.011 vs. HS group and P = 0.02 vs. Kae T group).

Conclusions

Pretreatment of hemorrhagic shock mice with kaempferol significantly decreased plasma levels of TNF-α and IL-6; reverted MPO, SOD, and MDA in the heart, lung, and liver; and increased expression of HO-1 in the same organs.

【 授权许可】

   
2015 Yang et al.; licensee BioMed Central.

【 图 表 】

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