The performance of the SD BIOLINE Dengue DUO® rapid immunochromatographic test kit for the detection of NS1 antigen, IgM and IgG antibodies during a dengue type 1 epidemic in Jamaica

Journal of Biomedical Science

John F. Lindo²  Marion Bullock DuCasse¹  Kereann Nelson²  Michelle G. Brown²  Kevin M. Harvey¹  Ivan E. Vickers² 

[1] Ministry of Health, Kingston, Jamaica;Department of Microbiology, The University of the West Indies, Kingston 7, Mona, Jamaica

关键词: Negative predictive value;    Positive predictive value;    Specificity;    Sensitivity;    Antigen;    Antibody;    NS1;    IgG;    IgM;    Dengue;   

Others  :  1219611
DOI  :  10.1186/s12929-015-0164-9

 received in 2015-02-16, accepted in 2015-07-03,  发布年份 2015

【 摘 要 】

Background

Dengue is an important mosquito-borne viral infection that affects millions of persons worldwide. Early diagnosis is necessary to effect appropriate management and decrease mortality. Immunochromatographic tests are advantageous in producing dengue test results within 30 min but these results should be sensitive and specific. In this study we evaluated the diagnostic performance of the SD BIOLINE Dengue DUO® rapid immunochromatographic test kit. A panel of 309 dengue and 30 non-dengue single serum samples characterized by using reference enzyme-linked immunosorbent assays (ELISAs) was used. These samples were received in the virology laboratory for routine testing during a dengue type 1 outbreak between October to December, 2012.

Results

The overall diagnostic sensitivities of the SD BIOLINE Dengue DUO® rapid testfor IgM, IgG and NSI were 49.3 % (95 % CI: 41.3-57.4), 39.1 % (95 % CI: 33.3-45.2) and 90 % (95 % CI: 82.1-94.7), respectively. The IgM and IgG detection rates were significantly lower than that of the NSI (p < 0.001). However the combination of the IgM detection with NS1 detection or both NS1 and IgG resulted in a significant (p < 0.001) increase in sensitivity to 97.5 % (95 % CI: 92.9-99.2) and 98.9 % (95 % CI: 96.0-99.7), respectively. These higher sensitivities were achieved without any decrease in specificities.

Conclusions

This study revealed that combining two or more parameters of the SD BIOLINE Dengue DUO® rapid kit significantly improved the sensitivity of diagnosis of dengue virus infection and supports its usefulness in the Jamaican setting.

【 授权许可】

   
2015 Vickers et al.

【 图 表 】

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