Mesenchymal stem cells overexpressing Ihh promote bone repair

Journal of Orthopaedic Surgery and Research

Hongliang Hu¹  Lixin Kan²  Zheng Li¹  Yiran Huang¹  Xizhi Guo³  Yong Zhu¹  Shi Yang¹  Pingping Song¹  Lingling Zhang³  Ruhui Tian¹  Yanan Wang¹  Tingting Chen¹  Shasha Zou¹ 

[1] Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 845 Lingshan Road, Shanghai 200135, China;Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago 60611, IL, USA;BIO-X Center, Shanghai Jiao Tong University, 55 Guangyuan West Road, Shanghai 200240, China

关键词: Tissue engineering;    Mesenchymal stem cells (MSCs);    Angiogenesis;    Osteogenesis;    Chondrogenesis;    Indian hedgehog (Ihh);   

Others  :  1151668
DOI  :  10.1186/s13018-014-0102-7

 received in 2014-06-23, accepted in 2014-10-10,  发布年份 2014

【 摘 要 】

Background

Indian hedgehog (Ihh) signaling pathway is known to play key roles in various aspects of normal endochondral bone development. This study tested the potential roles of high Ihh signaling in the context of injury-induced bone regeneration.

Methods

A rabbit tibia defect model was established to test the effects of the implant of Ihh/mesenchymal stem cells (MSCs)/scaffold complex. Computed tomography (CT), gross observation, and standard histological and immunohistological techniques were used to evaluate the effectiveness of the treatment. In vitro studies with MSCs and C3H10T1/2 cells were also employed to further understand the cellular and molecular mechanisms.

Results

We found that the implanted Ihh/MSCs/scaffold complex promoted bone repair. Consistently, in vitro study found that Ihh induced the upregulation of chondrocytic, osteogenic, and vascular cell markers, both in C3H10T1/2 cells and MSCs.

Conclusions

Our study has demonstrated that high Ihh signaling in a complex with MSCs enhanced bone regeneration effectively in a clinically relevant acute injury model. Even though the exact underlying mechanisms are still far from clear, our primary data suggested that enhanced chondrogenesis, osteogenesis, and angiogenesis of MSCs at least partially contribute to the process. This study not only has implications for basic research of MSCs and Ihh signaling pathway but also points to the possibility of direct application of this specific paradigm to clinical bone repair.

【 授权许可】

   
2014 Zou et al.; licensee BioMed Central Ltd.

【 图 表 】

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