Evaluation of oral mucosal epithelium in diabetic male patients by exfoliative cytology method

Journal of Diabetes & Metabolic Disorders

Babak Zamani⁴  Mohammad Mehdizadeh¹  Zoleikhah Moazzezi⁵  Farideh Feizi²  Safoura Seifi³ 

[1] Department of Oral and Maxillofacial Surgery, Babol Dental University, Babol, Iran;Department of Histology, Babol Medical University, Babol, Iran;Department of Oral and Maxillofacial Pathology, Babol Dental University, Babol, Iran;Dentist, Babol, Iran;Department of endocrinology, Babol Medical University, Babol, Iran

关键词: Oral exfoliative cytology;    Cytomorphomerry;    Diabetes mellitus;   

Others  :  1136138
DOI  :  10.1186/2251-6581-13-77

 received in 2013-09-02, accepted in 2014-06-29,  发布年份 2014

【 摘 要 】

Background

The goal of present study is to examine comparatively the epithelium of oral mucosa in persons with diabetes types I and II and the healthy persons by cytomorphometric method.

Methods

Cytology smear was prepared from oral mucosa epithelium in 24 persons with diabetes and 30 healthy persons were stained by papanicolao method. Its before, from patients were requested acquiring written. The quantitative cytomorphometric characteristics were measured in each group by motic plus 2 software. Also, the qualitative evaluation of cytology slides are performed in three groups.

Results

There were decrease in the nuclear and cytoplasmic size, (P < 0.001) and increase in the ratio of nuclear to cytoplasm size in buccal mucosal areas (P = 0.001) and tongue (P = 0.011) of diabetic persons compared to the healthy persons. There was no significantly statistical difference in diabetic persons types I and II in buccal mucosal area (P = 0.15) and tongue (P = 0.86) in quantitative characteristics of cytomoerphometry. In nuclear and cytoplasm size, there was a significant statistical difference in persons with diabetes type I and persons of control I and persons with diabetes type II and persons of control II in buccal mucosa and the tongue (P < 0.001). Bilobed or multi-lobed nuleous, karyorrhexis and vacuolization of cytoplasm were more in diabetic persons compared to the healthy ones (P < 0.001).

Conclusion

Diabetes are effective in creating some quantitative and qualitative cytometric alterations in the oral mucosa but the type of diabetes doesn’t seem to be effective on these changes.

【 授权许可】

   
2014 Seifi et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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