Autophagy-related gene 7 is downstream of heat shock protein 27 in the regulation of eye morphology, polyglutamine toxicity, and lifespan in Drosophila

Journal of Biomedical Science

Horng-Dar Wang¹  Guang-Chao Chen⁴  Pei-Yu Wang³  Chao-Yung Wang⁵  Chun-Pu Lin¹  Wan-Hua Li²  Cheng-Wen Huang³  Hong-Wen Tang⁴  Yi-Chun Chen²  Ming-Lun Kang²  Shih-Fen Chen² 

[1] Department of Life Science, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, HsinChu, 30013, Taiwan;Institute of Biotechnology, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, HsinChu, 30013, Taiwan;Institute of Neuroscience, National Chengchi University, 64, Section 2, Zhi-Nan Road, Taipei, 11605, Taiwan;Institute of Biological Chemistry, 128, Section 2, Academia Road, Nankang, Taipei, 115, Taiwan;Second Section of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Chang Gung University College of Medicine, Taoyuan, Taiwan

关键词: Drosophila;    Lifespan;    Neurodegeneration;    Hsp27;    Atg7;   

Others  :  825023
DOI  :  10.1186/1423-0127-19-52

 received in 2012-02-02, accepted in 2012-05-23,  发布年份 2012

【 摘 要 】

Background

Autophagy and molecular chaperones both regulate protein homeostasis and maintain important physiological functions. Atg7 (autophagy-related gene 7) and Hsp27 (heat shock protein 27) are involved in the regulation of neurodegeneration and aging. However, the genetic connection between Atg7 and Hsp27 is not known.

Methods

The appearances of the fly eyes from the different genetic interactions with or without polyglutamine toxicity were examined by light microscopy and scanning electronic microscopy. Immunofluorescence was used to check the effect of Atg7 and Hsp27 knockdown on the formation of autophagosomes. The lifespan of altered expression of Hsp27 or Atg7 and that of the combination of the two different gene expression were measured.

Results

We used the Drosophila eye as a model system to examine the epistatic relationship between Hsp27 and Atg7. We found that both genes are involved in normal eye development, and that overexpression of Atg7 could eliminate the need for Hsp27 but Hsp27 could not rescue Atg7 deficient phenotypes. Using a polyglutamine toxicity assay (41Q) to model neurodegeneration, we showed that both Atg7 and Hsp27 can suppress weak, toxic effect by 41Q, and that overexpression of Atg7 improves the worsened mosaic eyes by the knockdown of Hsp27 under 41Q. We also showed that overexpression of Atg7 extends lifespan and the knockdown of Atg7 or Hsp27 by RNAi reduces lifespan. RNAi-knockdown of Atg7 expression can block the extended lifespan phenotype by Hsp27 overexpression, and overexpression of Atg7 can extend lifespan even under Hsp27 knockdown by RNAi.

Conclusions

We propose that Atg7 acts downstream of Hsp27 in the regulation of eye morphology, polyglutamine toxicity, and lifespan in Drosophila.

【 授权许可】

   
2012 Chen et al.; licensee BioMed Central Ltd.

【 图 表 】

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