A longitudinal survey of African swine fever in Uganda reveals high apparent disease incidence rates in domestic pigs, but absence of detectable persistent virus infections in blood and serum

BMC Veterinary Research

Karl Ståhl⁶  Mikael Berg⁷  Michael Ocaido²  Richard P Bishop⁴  Rose Okurut Ademun⁸  Lawrence Mayega¹  Sofia Boqvist⁷  Ulf Emanuelson⁵  Charles Masembe³  Denis Muhangi² 

[1] District Veterinary Office, under the Ministry of Agriculture, Animal Industry and Fisheries, Masaka, Uganda;Department of Wildlife and Aquatic Resources, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda;Department of Biological Sciences, College of Natural Sciences, Makerere University, Kampala, Uganda;International Livestock Research Institute (ILRI), Nairobi, GPO 00100, Kenya;Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, SE-750 07, Sweden;Department of Disease Control and Epidemiology, National Veterinary Institute (SVA), Uppsala, SE-751 89, Sweden;Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, SE-750 07, Sweden;Ministry of Agriculture, Animal Industry and Fisheries, Entebbe, Uganda

关键词: Smallholder farmers;    Risk factors;    Incidence rate;    Epidemiology;    African swine fever (ASF);   

Others  :  1206135
DOI  :  10.1186/s12917-015-0426-5

 received in 2014-09-02, accepted in 2015-05-05,  发布年份 2015

【 摘 要 】

Background

African swine fever (ASF) is a fatal, haemorrhagic disease of domestic pigs, that poses a serious threat to pig farmers and is currently endemic in domestic pigs in most of sub-Saharan Africa. To obtain insight into the factors related to ASF outbreaks at the farm-level, a longitudinal study was performed in one of the major pig producing areas in central Uganda. Potential risk factors associated with outbreaks of ASF were investigated including the possible presence of apparently healthy ASF-virus (ASFV) infected pigs, which could act as long-term carriers of the virus. Blood and serum were sampled from 715 pigs (241 farms) and 649 pigs (233 farms) to investigate presence of ASFV and antibodies, during the periods of June-October 2010 and March-June 2011, respectively. To determine the potential contribution of different risks to ASF spread, a questionnaire-based survey was administered to farmers to assess the association between ASF outbreaks during the study period and the risk factors.

Results

Fifty-one (21 %) and 13 (5.6 %) farms reported an ASF outbreak on their farms in the previous one to two years and during the study period, respectively. The incidence rate for ASF prior to the study period was estimated at 14.1 per 100 pig farm-years and 5.6 per 100 pig farm-years during the study. Three pigs tested positive for ASFV using real-time PCR, but none tested positive for ASFV specific antibodies using two different commercial ELISA tests.

Conclusions

There was no evidence for existence of pigs that were long-term carriers for the virus based on the analysis of blood and serum as there were no seropositive pigs and the only three ASFV DNA positive pigs were acutely infected and were linked to outbreaks reported by farmers during the study. Potential ASF risk factors were present on both small and medium-scale pig farms, although small scale farms exhibited a higher proportion with multiple potential risk factors (like borrowing boars for sows mating, buying replacement from neighboring farms without ascertaining health status, etc) and did not implement any biosecurity measures. However, no risk factors were significantly associated with ASF reports during the study.

【 授权许可】

   
2015 Muhangi et al.; licensee BioMed Central.

【 图 表 】

Fig. 1.

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