D2-like receptor activation does not initiate a brain docosahexaenoic acid signal in unanesthetized rats

BMC Neuroscience

Epolia Ramadan¹  Stanley I Rapoport¹  Mei Chen¹  Lisa Chang¹  Ameer Y Taha¹ 

[1] Brain Physiology and Metabolism Section, Laboratory of Neuroscience, National Institute on Aging, National Institutes of Health, Bldg. 9, Room 1S126, Bethesda, MD 20892, USA

关键词: Arachidonic acid;    Calcium-dependent phospholipase A₂ (cPLA₂);    Calcium-independent phospholipase A₂ (iPLA₂);    Intracellular calcium;    Metabolism;    Signaling;    Release;    Docosahexaenoic acid;    Neurotransmission;    Quinpirole;    Dopamine;   

Others  :  1090759
DOI  :  10.1186/1471-2202-15-113

 received in 2014-05-11, accepted in 2014-09-23,  发布年份 2014

【 摘 要 】

Background

The polyunsaturated fatty acid, docosahexaenoic acid (DHA), participates in neurotransmission involving activation of calcium-independent phospholipase A₂ (iPLA₂), which is coupled to muscarinic, cholinergic and serotonergic neuroreceptors. Drug induced activation of iPLA₂ can be measured in vivo with quantitative autoradiography using ¹⁴C-DHA as a probe. The present study used this approach to address whether a DHA signal is produced following dompaminergic (D)2-like receptor activation with quinpirole in rat brain. Unanesthetized rats were infused intravenously with ¹⁴C-DHA one minute after saline or quinpirole infusion, and serial blood samples were collected over a 20-minute period to obtain plasma. The animals were euthanized with sodium pentobarbital and their brains excised, coronally dissected and subjected to quantitative autoradiography to derive the regional incorporation coefficient, k*, a marker of DHA signaling. Plasma labeled and unlabeled unesterified DHA concentrations were measured.

Results

The incorporation coefficient (k*) for DHA did not differ significantly between quinpirole-treated and control rats in any of 81 identified brain regions. Plasma labeled DHA concentration over the 20-minute collection period (input function) and unlabeled unesterified DHA concentration did not differ significantly between the two groups.

Conclusion

These findings demonstrate that D2-like receptor initiated signaling does not involve DHA as a second messenger, and likely does not involve iPLA₂ activation.

【 授权许可】

   
2014 Taha et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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